Banca de QUALIFICAÇÃO: DANIEL CHAVES DE LIMA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE: DANIEL CHAVES DE LIMA
DATA: 20/11/2015
HORA: 14:00
LOCAL: Sala Carl Peter von Dietrich - Depto. de Bioquímica - CB/UFRN
TÍTULO:

New findings in the biology of Chromobacterium violaceum:

homologous recombination and a new episome

PALAVRAS-CHAVES:

Homologous Recombination, RadA/Sms, RecA, Chromobacterium

violaceum.

PÁGINAS: 100
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
SUBÁREA: Biologia Molecular
RESUMO:

Chromobacterium violaceum is a ß-proteobacteria commonly found

around tropical and subtropical regions throughout the globe. This

specie has many metabolites with biotechnological properties such

as antitumoral peptides, antibiotics, polymers that have the potential

to replace the oil-based ones among others. Although it has been

extensively studied the past 40 years, there are many aspects of C.

violaceum that remains untouched until today. We have conducted a

biochemical overview on the homologous recombination (HR)

machinery of C. violaceum, most of the work based mainly in RecA

and its paralog, RadA. We performed in vitro reactions from initial

and late steps from homologous recombination such as D-loop

formation and branch migration, respectively, with their respective

molecular actors and how RadA influenced each one. We observed

that cvRadA influences, negatively, D-loop formation promoted by

cvRecA and from pull-down assay we predicted an interaction

between these two proteins. We also observed the DNA-binding

preference of cvRadA (and cvRecA) and observed that this protein

binds preferentially to dsDNA instead ssDNA, the opposite from cvRecA. No involvement of cvRadA on branch migration reactions

was detected. In this work, we also described the isolation, sequence

and annotation of a new plasmid, never detected before, from C.

violaceum, named ChVi1. It has 44,236 base pairs, 39 predicted

open reading frames and, possibly, two origins of replication. Most of

the ORFs codes for hypothetical and structural bacteriophage

proteins.

MEMBROS DA BANCA:
Interno - 1149647 - LUCYMARA FASSARELLA AGNEZ
Externo ao Programa - 2085604 - SUSANA MARGARIDA GOMES MOREIRA
Externo ao Programa - 032.540.854-85 - TIRZAH BRAZ PETTA LAJUS - UFRGS