Banca de QUALIFICAÇÃO: NEDNALDO DANTAS DOS SANTOS

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE: NEDNALDO DANTAS DOS SANTOS
DATA: 13/02/2012
HORA: 09:00
LOCAL: Sala de Aula do Departamento de Bioquímica - UFRN
TÍTULO:

ANTIPROLIFERATIVE ACTIVITY OF FUCAN NANOGEL

PALAVRAS-CHAVES:

nanoparticles; Cancer cell; Sulfated Polysaccharides, Spatoglossum schröederi

PÁGINAS: 11
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Sulfated fucans comprise families of polydisperse natural polysaccharides based on sulfated L-fucose. Our aim was to investigate whether fucan nanogel induces any cell-specific response. Therefore, a non toxic fucan extracted from Spatoglossum schröederi was chemically modified by the grafting hexadecylamine to the polymer hydrophilic backbone. The resulting modified material (SNFuc-TBa125) formed nanosized particles. ¹H NMR showed a substitution degree of the hydrophobic chains around 93%.  SNFfuc-TBa125 in aqueous media had a mean diameter of 123 nm and zeta potential of -38,3 ± 0.74 mV as measured bydynamic light scattering. The nanoparticles conserved their size for up to 70 days. SNFfuc-TBa125 cytotoxicity was determined using MTT assay after culturing different cell lines for 24h. Tumor-cell (HepG2, 786, H-S5) proliferation was inhibited by 2.0 – 43.7% at nanogel concentrations of 0.05– 0.5 mg/ mL; the RAEC non-tumor cell line proliferation was also inhibited by 8.0–22.0%. On the other hand, the CHO and RAW non-tumor cell lines proliferation was improved by the nanogel in the same concentration range. Flow cytometric analysis revealed that this fucan nanogel inhibited 786 cell proliferation through caspase and caspase-independent mechanisms. In addition, SNFfuc-TBa125 blocks 786 cell passages in the S phase of the cell cycle.

MEMBROS DA BANCA:
Interno - 347652 - CLODOMIRO ALVES JUNIOR
Presidente - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externo ao Programa - 2477216 - NAISANDRA BEZERRA DA SILVA