Banca de QUALIFICAÇÃO: JEFFERSON ROMÁRYO DUARTE DA LUZ

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : JEFFERSON ROMÁRYO DUARTE DA LUZ
DATA : 19/08/2016
HORA: 14:00
LOCAL: Sala de Reuniões do CONSEC/CCS
TÍTULO:

Extract and Ethyl Acetate Fraction of Licania rigida Benth has a Potential Anticoagulant Effect by Factor IIa Inhibition

PALAVRAS-CHAVES:

Licania rigida Benth; Toxicity; Coagulation

PÁGINAS: 35
RESUMO:

Ethnopharmacological relevance: Cardiovascular diseases are the main causes of mortality and morbidity worldwide. Anticoagulants are used as a secondary prevention of thromboembolic events. The use of plants for medicinal purposes is already a global practice. Licania rigida Benth (Chrysobalanaceae Family) is a tree of large and evergreen foliage, found in semi-arid regions of Brazil.

Aim of the study: To evaluate the toxicological risks in vitro and in vivo, as well as the in vitro anticoagulant potential of the crude extract (CELR) and ethyl acetate fraction (AFLR) of L. rigida leaves.

Materials and Methods: In vitro toxicity was assessed by an MTT assay in 3T3 and HEK-293 cells. An acute oral toxicity test was performed in Wistar rats. Toxicity was estimated by the determination of hematological and biochemical parameters related to liver and kidney functions. The anticoagulant ability of the extracts was measured by the aPTT, PT, and inhibition of Xa and IIa factors tests.

Results: In relation to the toxic effects, no toxicity was observed after exposure to the extracts. The anticoagulant potential was demonstrated in the aPTT and PT tests where the two extracts have the ability to double the standard coagulation time at the concentration of 50 µg/mL. However, only the AFLR was capable of inhibiting the extrinsic pathway of coagulation by inhibiting 100% of the IIa factor at a concentration of 100 µg/mL, compared with that by the clinical anticoagulant, heparin.

Conclusions: AFLR demonstrated an anticoagulant ability by inhibiting the extrinsic pathway of blood coagulation without causing toxic damage to the body and can become a target for further studies to validate its potential as a phytomedication.

MEMBROS DA BANCA:
Externo ao Programa - 1640129 - ADRIANA DA SILVA BRITO
Presidente - 346847 - MARIA DAS GRACAS ALMEIDA
Interno - 1490222 - SILVANA MARIA ZUCOLOTTO LANGASSNER