Banca de QUALIFICAÇÃO: ALLANNY ALVES FURTADO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE: ALLANNY ALVES FURTADO
DATA: 02/09/2015
HORA: 09:00
LOCAL: SALA DE AULA II DO PPGCF
TÍTULO:

EVALUATION OF ANTI-INFLAMMATORY PROPERTIES OF AQUEOUS EXTRACT FROM Ipomoea asarifolia (Desr.) Roem. and Schult LEAVES IN EXPERIMENTAL MURINE MODELS

PALAVRAS-CHAVES:

Ipomoea asarifolia, anti-inflammatory, zymosan, carrageenan, xylene

PÁGINAS: 50
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Abstract
Ethnopharmacological relevance
Ipomoea asarifolia (Desr.) Roem. and Schult.(Convolvulaceae), popularly known as salsa or salsa-brava, is a plant used in folk medicine to treat various inflammatory disorders such as oftalmias, dysmenorrhea, syphilis and other inflammatory processes related to the genital tract; it is also used for gastrointestinal disorders, wound healing, diabetes, among others. However, to date, no study has shown possible compounds and mechanisms of action of the plant to support the activities reported by popular use.
Aim of the study
The study aimed to identify bioactive molecules present in the crude extract of I. asarifolia leaves and investigate the anti-inflammatory potential of this extract in different experimental models, as well as to verify whether the identified compounds presented activity in the tested models.
Material and methods
Aqueous extract of I. asarifolia leaves was prepared by decoction and the compounds were identified by high performance liquid chromatography coupled with diode array detector (HPLC-DAD). The potential anti-inflammatory activity of the extract was assessed using the following in vivo models: xylene-induced ear edema, evaluating the degree of edema formation; carrageenan-induced peritonitis, evaluating leukocyte migration and cytokine levels (IL-1β, IL-6, IL-12 and TNF-α) at 4 hours; zymosan-induced air pouch, evaluating the kinetics of leucocyte migration by total and differential counts at 6, 24 and 48 hours. The same tests were conducted for the compounds identified in the aqueous extract from I. asarifolia leaves.
Results
The compounds identified in the aqueous extract of I. asarifolia leaves by high HPLC-DAD were rutin, chlorogenic acid and caffeic acid. The extract demonstrated the ability to significantly inhibit the production of cytokines IL-1β, IL-6, IL-12 and TNF-α. In addition, significantly reduced ear edema induced by xylene and cell migration in experimental models of peritonitis and air pouch. The secondary metabolites tested also showed the ability to significantly decrease the parameters analyzed above.
Conclusion
This study made possible to assess the anti-inflammatory potential of I. asarifolia leaves and identify biomolecules present in the extract, suggesting that these compounds are contributing to this activity supported by popular use. By means of different experimental models, it was concluded that the plant might be used as a source for the development of phytomedicines for the treatment of inflammatory disorders.

MEMBROS DA BANCA:
Interno - 1517215 - ALINE SCHWARZ
Externo à Instituição - SARAH DANTAS VIANA MEDEIROS - FANEC
Presidente - 1490222 - SILVANA MARIA ZUCOLOTTO LANGASSNER