Banca de QUALIFICAÇÃO: JOAO VICTOR VILLAS BOAS SPELTA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : JOAO VICTOR VILLAS BOAS SPELTA
DATE: 04/03/2024
TIME: 16:00
LOCAL: Google Meet, meet.google.com/eqe-fijh-ise
TITLE:

GENIC PREDICTION RELATED TO SEXUAL DETERMINATION IN CARICA PAPAYA (CARICACEAE)

KEY WORDS:

Hermaphrodite; GWAS; genome

PAGES: 30
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Papaya (Carica papaya) produces one of the most consumed fruits in the world. The papaya cultivators use gyno-dioecious plants and, for its cultivation to be more efficient, requires a greater proportion of hermaphrodite plants, considering that fruits from female and male papaya trees are overlooked by consumer markets. The seeds available on the market generate plants of all three types, male, female and hermaphrodite, and the lack of strategies other than molecular markers still represents a limitation in its production. Knowing the molecular bases that influence the mechanism of sexual determination in papaya trees can help in the development of strategies that assist in the selection of hermaphrodite plants. In this work, a bioinformatics strategy was used to address the topic. An association study between genotypes and phenotypes was then carried out to find possible genetic factors involved in sexual determination using resequencing data from 36 samples of mothers (24 males and 12 hermaphrodites) obtained from public databases after a setp of variant calling was carried out in bcftools. 136,916 variants were found, leaving 41,375 after filtering. The association was then carried out using the PLINK program with these variants and among these, 159 of the most significant ones were submitted to SnpEff, resulting in the prediction of 767 effects, including 751 with a modifier impact effect, 7 with low impact effect and 9 with a moderate. Blastsp were then carried out in NCBI with the sequences of genes that had moderate and low effects and their results were recorded. This work was compared with what has already been done in the literature and it is concluded that the results identified so far are a starting point for more in-depth studies to progress in understanding the molecular mechanisms of sexual determination in C. papaya.

COMMITTEE MEMBERS:
Externo ao Programa - 2257836 - PAULO SERGIO MARINHO LUCIO - nullPresidente - 3063244 - TETSU SAKAMOTO
Externa ao Programa - 3330361 - VASILIKI LAGOU - null