Banca de DEFESA: THAÍS DE ALMEIDA RATIS RAMOS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : THAÍS DE ALMEIDA RATIS RAMOS
DATE: 02/08/2022
TIME: 15:00
LOCAL: https://meet.google.com/obp-nxfb-gmo
TITLE:

SSingle cell systems biology of long non-coding RNAs associated with cardiac tissue development and cardiovascular disease

KEY WORDS:

Long non-coding RNAs, Single-cell, Heart development, Cardiovascular disease, Machine learning, Cardiomyocyte subpopulation.

PAGES: 133
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Long non-coding RNAs (lncRNAs) comprise the most representative transcriptional units of the mammalian genome, and they’re associated with organ development that can be associated with the emergence of diseases, such as cardiovascular diseases. The World Health Organization (WHO), for example, has published that cardiovascular diseases are responsible for the death of 17.9 million people each year, corresponding to 31% of all deaths all around the world. Therefore, a combination of transcripts from Gencode (M20), Ensembl (GRCm38.95) and Amaral et al. (2018) databases was used to define the set of non-redundant reference lncRNAs; and Gencode (M20) for the reference coding transcripts. In addition, bioinformatics approaches, machine learning algorithms and statistical techniques were used to define lncRNAs involved in mammalian cardiac development in a single-cell perspective. For this, the single-cell database published by DeLaughter et al. (2016) was used, in which there were data from 4 embryonic stages (E9.5, E11.5, E14.5, E18.5) and 4 post -natals (P0, P3, P7, P21) of the mus musculus model organism. Our study identified 8 distinct cell types, novel marker transcripts (coding/lncRNAs) and also, differential expression and functional enrichment analysis revealed cardiomyocyte subpopulations associated with cardiac function; meanwhile modular co-expression analysis reveals cell-specific functional insights for lncRNAs during myocardial development, including a potential association with key genes related to disease and the “fetal gene program”. Our results evidence the role of particular lncRNAs in heart development, and highlights the usage of co-expression modular approaches in the cell-type functional definition. As future work, we intend to identify the functional roles of these RNAs in the development of cardiac tissues and in cardiovascular diseases using experimental validation approaches.

COMMITTEE MEMBERS:
Presidente - ***.739.204-** - VINICIUS RAMOS HENRIQUES MARACAJA COUTINHO - USP
Interno - 1507794 - RODRIGO JULIANI SIQUEIRA DALMOLIN
Interna - ***.117.554-** - THAIS GAUDENCIO DO REGO - UFPB
Externo à Instituição - GILDERLANIO SANTANA DE ARAÚJO - UFPA
Externo à Instituição - YURI DE ALMEIDA MALHEIROS BARBOSA