Banca de QUALIFICAÇÃO: LUCAS FELIPE DA SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : LUCAS FELIPE DA SILVA
DATE: 30/03/2021
TIME: 14:00
LOCAL: remoto plataforma google meet
TITLE:

BIOINFORMATIC APPROACHES APPLIED IN THE ANALYSIS OF DATA GENERATED BY ABIOTIC STRESS: MICROGRAVITY AND HYDROGEN PEROXIDE IN SUGARCANE PLANTS

KEY WORDS:

VSB-30 sounding rocket, microgravity, RNA-seq, oxidative stress, transcriptome, enrichment, Saccharum spp.

PAGES: 63
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Biologia Molecular
SUMMARY:

Sugarcane (Saccharum spp.) is a monocot of the Poaceae family, a C4 plant, adapted to tropical and subtropical regions and with a high level of polyploidy due to interspecific crossing. Brazil is the largest world producer. As a consequence of a sessile nature, plants are exposed to different biotic and abiotic factors that may promote an oxidative stress condition. However, cells have different antioxidant mechanism to keep their homeostasis and reduce ROS levels. It has been observed that modifications on gravitational field may trigger responses in plants similar to the abiotic stress responses. In the present work, RNA sequencing data from sugarcane plants submitted to the VSB-30 sounding rocket flight were analyzed. This material was subjected to six minutes of microgravity. Thus, using bioinformatics approaches, this work aimed to identify genes that showed differential expression under the condition of microgravity in roots and leaves submitted in horizontal and vertical orientations. For this, the Sorghum bicolor species was adopted as the reference genome for the alignment and the species Zea mays, Oryza sativa and other grasses were used to obtain homology description of uncharacterized genes. The results of this bioinformatics analysis showed which were the unique and specific genes for each of the nine data libraries analyzed, such as the genes C5YT78, C5YWA7 and C5WLY2, per example. It was also identified which were the enriched metabolic pathways in response to the microgravity condition, such as: Phosphatidylinositol signaling system and Amino sugar and nucleotide sugar metabolism. Thus, the results obtained with bioinformatics approaches allowed a better understanding of the loss of tissue structural organization after the recovery of this material, in addition to understand to the accumulation of lignin, H2O2 and EROs. Moreover, these results from histology, biochemistry and RNAseq analysis allowed to observe that the alteration of gravity was perceived by the sugarcane plants (leaves and roots) and translated signaling through reactive oxygen species under stress conditions and this expression were correlated to the different metabolic pathways observed, for example, the Pentose phosphate pathway and the RNA transport. This analysis allowed to correlated the molecular data (expression) with histological and biochemical data.  In addition, to understand how leaves and roots might perceive the alteration and trigger the molecular response for the horizontal or vertical plants subjected to microgravity. In conclusion, the present work allowed the identification of genes, active metabolic pathways and understanding of the different responses of the sugarcane plant under the gravity changes with the flight of the VSB-30 sounding rocket.

BANKING MEMBERS:
Presidente - 1453487 - KATIA CASTANHO SCORTECCI
Interno - 1513597 - JOAO PAULO MATOS SANTOS LIMA
Externo à Instituição - CARLOS HENRIQUE SALVINO GADELHA MENESES - UEPB