Banca de QUALIFICAÇÃO: RENATA LILIAN DANTAS CAVALCANTE

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : RENATA LILIAN DANTAS CAVALCANTE
DATE: 13/03/2020
TIME: 13:00
LOCAL: BioME - PPg-Bioinfo
TITLE:

Exploratory investigation of genetic factors associated with the sexual determination system in Arapaima gigas (Pirarucu)

KEY WORDS:

Osteoglossiformes; Arapaima gigas; Sexual Differentiation

PAGES: 43
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

The Pirarucu, (Arapaima gigas) is one of the largest freshwater bony fish in the world, may weigh around 200 kilograms and measure about 3 meters as an adult. It belongs to the family Arapaimidae, order Osteoglossiformes and has the Amazon Basin as its natural habitat. Due to its large size and its flesh containing low fat and low bone, breeding of Arapaima gigas has been increasingly motivated. A problem related to its fishery exploitation is that the genetic mechanisms that control the sexual differentiation in Arapaima gigas are not known, and your exual maturation occurs around the third to fifth year of life, and sexual dimorphism is not a strong characteristic of the species. For more sustainable management, it is of paramount importance to seek an effective and non-invasive method to sexually differentiate juvenile individuals. For this, the establishment of a molecular genetic marker related to sexual differentiation would be an advantageous tool. Previous analyses of the Arapaima gigas genome could not find significant results in determining genes or large genomic regions that are associated with the sex-determination system of these individuals. In this study, we proposed different approaches to identify repetitive regions in excess or lacking in one sex. For this purpose, we used genomic data from six adult representatives of Arapaima gigas, three males and three females. After these exploratory studies in the genome, it was noticed the existence of k-mers that are represented differently among individuals of the opposite sex. Not only the existence of k-mers but also, the identification of some scaffolds that the presence of haploid is present in one sex and with the opposite scenario in the other. The different proportions in the count of k-mers and heterozygous sites may indicate the existence of genetic factors that may aid in the sexing of individuals.

BANKING MEMBERS:
Presidente - 3063244 - TETSU SAKAMOTO
Interno - 2170415 - JORGE ESTEFANO SANTANA DE SOUZA