Banca de QUALIFICAÇÃO: CAYRO DE MACÊDO MENDES

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : CAYRO DE MACÊDO MENDES
DATA : 29/06/2018
HORA: 10:00
LOCAL: BioME
TÍTULO:

Characterization of putative proteins encoded by variable regions of white spot syndrome virus genome: an in silico approach

PALAVRAS-CHAVES:

Litopenaeus vannamei. Shrimp. Whispovirus. Ab initio.

PÁGINAS: 30
GRANDE ÁREA: Ciências Biológicas
ÁREA: Biologia Geral
RESUMO:

In silico characterization has been employed as a more accessible alternative for prediction of protein sequences that cannot be reproduced in vitro or have their structures crystallized, as well as can provide data that complement experimental approaches. The virus that causes white spot syndrome (WSSV) is one of the biggest problems facing global shrimp farming, causing considerable economic damage. Although the effects of the virus on the cultures are well known, to date there is little information on the mechanisms of viral infection and replication, mainly because much of their coding sequences do not show homology with known sequences. In addition, the WSSV genome has some coding regions that vary between the different isolates, which have not been functionally characterized to date, called ORF75, ORF94, ORF125, ORF23/24, ORF14/15. This work aimed at the in silico characterization of the putative proteins encoded by the variable regions of the WSSV genome, in order to identify possible functions. Phylogenetic analysis were performed from the alignment of ten WSSV genomic sequences obtained from GenBank. The variable regions of the ORF75, ORF94 and ORF125 were aligned and their repeat units and SNPs annotated through Geneious platform. The amino acid sequences were subjected to searches for remote homologous, motifs, conserved domains, fold recognition and prediction of secondary and tertiary structures. It was possible to model tertiary structures of protein domains and to infer possible functions that include an RNA recognition motif associated with post-transcriptional processes between positions 70-150 of wsv477 (ORF23), an Ankyrim repeat (ANK) motif acting in conjunction with RING-H2 domain on modulation of ubiquitin-dependent proteolysis in wsv249 (ORF125), repair helicases (wsv479, wsv497), actin filament polymerization associated protein (wsv463a), and a HA2 subunit of influenzavirus hemagglutinin subunit (wsv492 ). It has also been possible to detect signatures associated with nuclear localization signals within the repeating units of the amino acid sequences encoded by ORF75 and ORF94 which may be involved in the emission of signals to host cell transport proteins from nucleus. By means of these results an action model was proposed for each one of the proteins studied.

MEMBROS DA BANCA:
Presidente - 1880243 - DANIEL CARLOS FERREIRA LANZA
Interno - 1513597 - JOAO PAULO MATOS SANTOS LIMA