Banca de DEFESA: BEATRIZ SILVA LOPES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : BEATRIZ SILVA LOPES
DATE: 16/05/2024
TIME: 14:00
LOCAL: DNUT e google meet
TITLE:

TOXICOLOGICAL SCREENING OF HYDROALCOHOLIC EXTRACT OF JAMBOLÃO (Syzygium Cumini (L.) Skeels) IN ZEBRAFISH (Danio rerio)

KEY WORDS:

Myrtaceae; anthocyanins; biosecurity; development of toxicity; Brachydanio rerio

PAGES: 60
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUBÁREA: Bioquímica da Nutrição
SUMMARY:

Jambolão (Syzygium cumini (L.) Skeels) is an important source of bioactive compounds, particularly anthocyanins, a class of phenolic compounds that contribute to its biological properties. However, prior to evaluating its bioactive properties, it is necessary to conduct investigations that ensure safe dosages for its use. In this regard, this study aimed to perform a toxicological screening of jambolão hydroalcoholic extract (EJ) administered in different concentrations to using the zebrafish model (Danio rerio) at different life stages (embryonic and adult) of this organism. EJ was obtained from freeze-dried jambolão fruits, using 70% v/v ethanol through exhaustive extraction, and subjected to high performance liquid chromatography (HPLC) and characterized for total phenolic compounds (CFT), total monomeric anthocyanins (AMT) and antioxidant activity (ABTS and DPPH). Embryonic and adult zebrafish were exposed to serial concentrations of EJ (10 – 400 µg/ml) and subjected to acute toxicological screening, cardio and neurotoxicity evaluation. Fifteen phenolic compounds were identified by HPLC. The EJ showed a CFT content of 21.80 ± 0.01 mg EAG/g fruit and AMT 11.39 ± 0.91 mg Cy3-G/g fruit, with DPPH antioxidant activity of 844.70 ± 58.81 mmol Eq. Trolox/g and ABTS+ of 323.88 ± 11.05 mmol Eq. Trolox/g, with inhibitory activity (IC50) of ABTS+ at 0.606 mg/ml. EJ concentrations exceeding 50 µg/ml caused a greater number of malformations and death in both life stages of zebrafish. The estimated mean lethal concentration (LC50) for embryos was 118.4 ± 11.7 µg/ml and for adults it was 68.86 ± 4.45 µg/ml, with the chronic toxic dose estimated at 10.22 µg/ml ml and 5.54 µg/ml respectively. In general, there was no cardio or neurotoxicity observed in larvae exposed to EJ, but those exposed to a concentration of 10 µg/ml had lower behavioral performance (p < 0.0001). In adults, anxious behavior was noted in the EJ 10 µg/ml group in the new tank and avoidance tests (p < 0.05 and p ≥ 0.005), and in the EJ 100 µg/ml group in the sociability test (p < 0 .05); the EJ 50 µg/ml group had greater exploratory activity in the open field and aggressiveness tests (p < 0.05). Additionally, histopathological analyzes showed that animals in all groups, including the control group, presented mononuclear inflammatory infiltrate in the spinal cord, micro and macrovesicular steatosis, and intestine with compromised morphology. However, some animals in the groups exposed to EJ showed preserved intestinal morphology, suggesting a protective effect of EJ on this organ. Given the above, the results indicate that median doses of EJ (around 25 µg/ml) are safe in both life stages of zebrafish, however, new tests addressing aspects that were not analyzed in the present study need to be carried out at in order to guarantee the safety and effectiveness of EJ with a view to enabling the advancement of research into the bioactive potential of this extract in different health problems.

COMMITTEE MEMBERS:
Externa ao Programa - 1644341 - ANA CAROLINA LUCHIARI - nullExterno à Instituição - FABIANO PERES MENEZES
Presidente - 1971809 - JULIANA KELLY DA SILVA MAIA
Interna - 2275877 - THAIS SOUZA PASSOS