Banca de DEFESA: ISABELLE LUNA DE OLIVEIRA DANTAS BERTO
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.STUDENT : ISABELLE LUNA DE OLIVEIRA DANTAS BERTO
DATE: 24/04/2024
TIME: 09:00
LOCAL: REMOTA - https://meet.google.com/ptn-pzob-xvt
TITLE:
TOWARD ENHANCED ANTIOXIDANT AND PROTECTIVE POTENTIAL: CONJUGATION OF CORN COB XYLAN WITH GALLIC ACID AS A NOVEL APPROACH
KEY WORDS:
Modified polysaccharides; oxidative stress; oxidative damage; zebrafish
PAGES: 47
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:
Maize ranks as the second most widely produced crop globally, yielding approximately 1.2 billion tons, with corn cob being its primary byproduct, constituting 18 kg per 100 kg of corn. Agricultural corn production generates bioactive polysaccharide-rich byproducts, including xylan (Xyl). In this study, we used the redox method to modify corn cob xylan with gallic acid, aiming to enhance its antioxidant and protective capacity against oxidative stress. The conjugation process resulted in a new molecule termed conjugated xylan–gallic acid (Xyl-GA), exhibiting notable improvements in various antioxidant parameters, including total antioxidant capacity (1.4-fold increase), reducing power (1.2-fold increase), hydroxyl radical scavenging (1.6-fold increase), and cupric chelation (27.5-fold increase) when compared with unmodified Xyl. At a concentration of 1 mg/mL, Xyl-GA demonstrated no cytotoxicity, significantly increased fibroblast cell viability (approximately 80%), and effectively mitigated intracellular ROS levels (reduced by 100%) following oxidative damage induced by H2O2. Furthermore, Xyl-GA exhibited non-toxicity toward zebrafish embryos, offered protection against H2O2-induced stress, and reduced the rate of cells undergoing apoptosis resulting from H2O2 exposure. In conclusion, our findings suggest that Xyl-GA possesses potential therapeutic value in addressing oxidative stress-related disturbances. Further investigations are warranted to elucidate the molecular structure of this novel compound and establish correlations with its pharmacological activities
COMMITTEE MEMBERS:
Externo à Instituição - CARLOS EDUARDO BEZERRA DE MOURA - UFERSA
Presidente - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externa ao Programa - 1720860 - VANESSA DE PAULA SOARES RACHETTI - null