Banca de QUALIFICAÇÃO: LUCAS AMARAL MACHADO
Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.DISCENTE : LUCAS AMARAL MACHADO
DATA : 29/03/2019
HORA: 09:30
LOCAL: sala de aula do PPGCSa
TÍTULO:
A TECHNOLOGICAL APPROACH TO INCREASE THE CYTOTOXIC EFFECT OF BULLFROG (Rana catesbeiana Shaw) OIL, in natura, AGAINST A2058 MELANOMA CELLS (AN in vitro EVIDENCE)
PALAVRAS-CHAVES:
Bullfrog oil, nanocapsules, nanoemulsion, microemulsion, anti-melanoma activity, cell necrosis.
PÁGINAS: 50
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:
The aim of this work was to investigate the in vitro cytotoxic effect of previously developed nanocapsules, nanoemulsion and microemulsion systems based on bullfrog oil (BFO) against human melanoma cells (A2058). The nanosystems were produced as described in previous studies and characterized according to droplet/particle distribution and zeta potential. The biocompatibility was evaluated by the determination of the hemolytic potential against human erythrocytes. The cytotoxicity assessment was made based on MTT and cell death assays, determination of the Reactive Oxygen Species (ROS) levels and cell uptake. The three nanosystems were successfully reproduced and showed hemolytic potential smaller than 10 % at all oil concentrations (50 and 100 µg.mL-1 ). The MTT assay revealed that the nanosystems decreased the mitochondrial activity up to 92 ± 2 %. The study showed that the free BFO induced cell apoptosis, while all nanostructured systems caused cell death by necrosis, associated to a ROS overproduction and increased ability to deliver the BFO across all cellular compartments (membrane, cytoplasm and nucleus). Finally, these results elucidate the in vitro BFO nanosystems cytotoxic effect against human melanoma cells, suggesting that these systems can be further investigated as safe and promising pharmaceutical products able to improve the therapeutic arsenal against melanoma.
MEMBROS DA BANCA:
Interno - 1639820 - ARNOBIO ANTONIO DA SILVA JUNIOR
Presidente - 1178187 - ERYVALDO SOCRATES TABOSA DO EGITO
Externa à Instituição - Nereide Stela Santos Magalhães - UFPE