Banca de QUALIFICAÇÃO: FRANCISCO ALEXANDRINO JÚNIOR

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : FRANCISCO ALEXANDRINO JÚNIOR
DATA : 03/06/2019
HORA: 15:00
LOCAL: Faculdade de Farmácia - Sala 2 do PPGCF
TÍTULO:

Development of therapeutic systems for cutaneous leishmaniasis: A physicochemical approach

PALAVRAS-CHAVES:

Amphotericin B; leishmaniasis; microemulsion; skin; stability  

PÁGINAS: 106
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

This thesis is part of the group's efforts to develop therapeutic alternatives containing amphotericin B (AmB) for the treatment of leishmaniasis. Since the instability of AmB in aqueous medium is a complex mechanism and it dependents on factors such as the state of aggregation, degree of ionization (pKa) and pH of the medium, the first chapter aimed to determine the pKa values of AmB in distinct hydroalcoholic solutions through the Bjerrum plot. Such approach provided support to elaborate a mathematical model with suitable predictive and explanatory capacity. Therefore, allowing a better understanding of the mechanisms involved in the aqueous instability of AmB. The second chapter aimed to evaluate the kinetic profiles of AmB released from polymeric systems with different geometry (fibers and films) and composition ((polyvinyl alcohol) (PVA) and poly (lactic acid) (PLA)). The correlation of such profiles with thermodynamic parameters sugested that the drug release was an endothermic and non-spontaneous process, with the kinetic profiles of fibers and films consistent with the Peppas-Sahlin and Higuchi model, respectively. Since the PVA hydrogels demonstrated a better control in the AmB release, their properties as a potential therapeutic system were evaluated in vitro in the third chapter. Wherein the results revealed that the system was able to control the water vapor permeation to levels compatible with the skin in its physiological state. Additionally, it displayed effective antifungal and anti-leishmania activity, without potential cytotoxicity against VERO cells. The fourth chapter reports the results obtained from the development and physichochemical evaluation and modeling of AmB-loaded thermo-responsive microemulsions (ME). In which the aqueous instability of AmB, observed in chapter one, was attenuated by adding poloxamer 407 into the system. Therefore, increasing the half-life of AmB and hindering instability phenomena caused by thermo-destabilization. Thus, the results obtained herein demonstrate that PVA hydrogels and thermo-responsive ME are potential systems to be used in the topical outpatient treatment of cutaneous leishmaniasis.

MEMBROS DA BANCA:
Externo à Instituição - ANSELMO GOMES DE OLIVEIRA - UNESP
Presidente - 1178187 - ERYVALDO SOCRATES TABOSA DO EGITO
Externo ao Programa - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA