Banca de QUALIFICAÇÃO: HAROLDO GURGEL MOTA FILHO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : HAROLDO GURGEL MOTA FILHO
DATA : 27/04/2018
HORA: 08:30
LOCAL: Departamento de Odontologia
TÍTULO:

Effect of low level laser therapy on cell viability and oxidative stress of periodontal ligament stem cells

PALAVRAS-CHAVES:

Stem Cells; Laser Therapy;Reactive Oxygen Epecies. 

PÁGINAS: 27
GRANDE ÁREA: Ciências Biológicas
ÁREA: Biologia Geral
RESUMO:

Low level laser therapy (LLLT) is widely used in different areas of the regenerative medicine, especially for its beneficial effects on cellular metabolism. This study aims to evaluate the effects of LLLT on cell viability and oxidative stress of periodontal ligament stem cells (PDLSCs). PDLSCs will be isolated from the periodontal ligament extracted from human teeth. The cells will be characterized, through the differentiation in adipogenic and osteogenic culture media and also analysis of surface markers of stem cells in flow cytometry. After their isolation and characterization, the PDLSCs will be maintained under cell culture conditions, and divided into three groups: Group I (control); Group II (Laser 7.5 J / cm 2) and Group III (Laser 22.5 J / cm 2). The experiments were performed in quadruplicate and occurred in three time intervals (T0h, T24h and T48h). After the intervals, the experimental tests were done to evaluate the cellular parameters. For the cellular viability analysis, the Alamar Blue tests, the MTT assay, and the evaluation in flow cytometry of Annexin V and PI will be used. To evaluate the release of reactive oxygen species, the dosage of malonaldehyde will be used. Preliminary results showed that the cells scored positively for markers of mesenchymal stem cells. The MTT result showed that Group III had a lower absorbance in the range of T48h with statistical difference in relation to both Group I and in relation to Group II.

MEMBROS DA BANCA:
Presidente - 1958729 - RUTHINEIA DIOGENES ALVES UCHOA LINS
Interno - 2085732 - ANA RAFAELA LUZ DE AQUINO MARTINS
Interno - 829.172.214-53 - ROSEANE CARVALHO VASCONCELOS - UFRN