Banca de DEFESA: RODRIGO MARQUES DE MELO SANTIAGO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : RODRIGO MARQUES DE MELO SANTIAGO
DATE: 07/09/2023
TIME: 14:00
LOCAL: ICe - Auditório e pelo link - https://meet.google.com/xmw-gqhu-cua
TITLE:

Classification of dentate spikes based on their waveforms

KEY WORDS:

current source density, dentate gyrus, dentate spike, entorhinal cortex, unsupervised classification, waveform

PAGES: 74
BIG AREA: Ciências Biológicas
AREA: Fisiologia
SUMMARY:

Synchronous excitatory discharges from the entorhinal cortex (EC) to the dentate gyrus (DG) generate fast and prominent patterns in the hilar local field potential (LFP), called dentate spikes (DSs). As sharp-wave ripples in CA1, DSs are more likely to occur in quiet behavioral states, when memory consolidation is thought to take place. However, their functions in mnemonic processes are yet to be elucidated. The classification of DSs into types 1 or 2 is determined by their origin in the lateral or medial EC, as revealed by current source density (CSD) analysis, which requires recordings from linear probes with multiple electrodes spanning the DG layers. To allow the investigation of the functional role of each DS type in recordings obtained from single electrodes and tetrodes, which are abundant in the field, we developed an unsupervised method using Gaussian mixture models to classify such events based on their waveforms. Our classification approach achieved high accuracies (> 80%) when validated in 8 mice with DG laminar profiles. The average CSDs, waveforms, rates, and widths of the DS types obtained through our method closely resembled those derived from CSD-based classification. As an example of application, we used the technique to analyze single-electrode LFPs from apolipoprotein (apo) E3 and apoE4 knock-in mice. We observed that the latter group, which serves as a model for Alzheimer’s disease, exhibited wider DSs of both types from a young age, with a larger effect size for DS type 2, likely reflecting early pathophysiological alterations in the EC-DG network, such as hyperactivity. In addition to the applicability of the method in expanding the study of DS types, our results show that their waveforms carry information about their origins, suggesting different underlying network dynamics and roles in memory processing.

COMMITTEE MEMBERS:
Presidente - 1721223 - ADRIANO BRETANHA LOPES TORT
Externo à Instituição - CLEITON LOPES AGUIAR - UFMG
Externo à Instituição - FLAVIO FREITAS BARBOSA - UFPB
Externo ao Programa - 2190521 - HINDIAEL AERAF BELCHIOR - nullExterno à Instituição - RENAN CIPRIANO MOIOLI - IINN