Banca de DEFESA: RODOLFO DIÓGENES DA PAIXÃO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : RODOLFO DIÓGENES DA PAIXÃO
DATE: 15/07/2021
TIME: 09:00
LOCAL: GOOGLE MEET - meet.google.com/etu-junk-yui
TITLE:

Effect of a Cannabis extract on noise induced tinnitus and anxiety-related behaviour in adolescent mice

KEY WORDS:

Tinnitus, Cannabis extract and anxiety

PAGES: 54
BIG AREA: Ciências Biológicas
AREA: Fisiologia
SUMMARY:

Tinnitus is an auditory condition characterised by phantom sounds described as ringing or hissing sounds. Chronic bothersome tinnitus often leads to stress, anxiety and even depression and there is a lack of standardised treatments, . Recently the endocannabinoid system has gathered attention as it might modulate behaviour and synaptic activity. However, literature is still inconclusive about beneficial or detrimental effects of Cannabis treatment for tinnitus. Here we used a model of noise- induced tinnitus to investigate firstly, if tinnitus is altered by low dose Cannabis extract (2.44 mg/kg; THC, 1.0 mg/kg), and secondly, if anxiety is related to tinnitus-like behaviour and if it is affected by Cannabis. We used the gap prepulse inhibition of the acoustic startle (GPIAS) to assess tinnitus perception and the open field (OF) and elevated plus maze (EPM) to evaluate anxiety behaviour. Our preliminary results show that we can detect tinnitus-like behaviour when subdividing noise-exposed mice (NE) into Exposed Tinnitus mice (ET) and Exposed No Tinnitus mice (ENT), but Cannabis treatment had no effect on tinnitus-like behaviour. Moreover, we investigated if anxiety-like behaviour was associated with noise exposure or tinnitus perception, and found that Cannabis treatment made NE mice enter the centre less (p = 0.0492) and decreased locomotion (p = 0.0416). In EPM, sham mice showed more arm entries independently of treatment (vehicle, p = 0.0416; Cannabis, p = 0.0047) while NE entered less in open arms after Cannabis (p = 0.0026). Also, NE spent more time in closed arms and less time in open arms after Cannabis (closed, p = 0.0043; open, p = 0.0272).   Subdivision of NE mice revealed, for OF, that Cannabis reduced centre entries for ET (p = 0.0317) while reduced time in centre for ENT (p = 0.0272). For EPM, ET showed less entries into arms (closed, p = 0.0256; open, p = 0.022) and spent more time in closed arms (p = 0.0094) after Cannabis. In summary this work shows that anxiety tests can be an addition to GPIAS test to evaluate tinnitus and therapeutical effects. However, future studies will test lower doses for potential anxiolytic effects of THC, in treating tinnitus-related stress and anxiety.

BANKING MEMBERS:
Interno - 1728817 - CLAUDIO MARCOS TEIXEIRA DE QUEIROZ
Presidente - 1976236 - EMELIE KATARINA SVAHN LEAO
Externo à Instituição - FLAVIO FREITAS BARBOSA - UFPB