Banca de DEFESA: EMANUELLY BERNARDES DE OLIVEIRA DA SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : EMANUELLY BERNARDES DE OLIVEIRA DA SILVA
DATE: 31/08/2020
TIME: 14:30
LOCAL: Videoconferência via MEJC
TITLE:

ANÁLISE DE PLASMA SANGUÍNEO POR ESPECTROSCOPIA ATR-FTIR:
IDENTIFICAÇÃO DE BIOMOLÉCULAS EM MULHERES COM DIABETES
MELLITUS GESTACIONAL E SUA IMPLICAÇÃO EM DESFECHOS
ADVERSOS NEONATAIS

KEY WORDS:

Gestational diabetes mellitus; ATR-FTIR spectroscopy; GA-LDA; Newborns outcomes; Macrosomy;

PAGES: 107
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Gestational Diabetes Mellitus (DMG) is a hyperglycemic imbalance,
which affects 5 - 25% of pregnancies worldwide. Currently, there is a lack of
consensus on the knowledge of the pathophysiology of DMG. Our study
proposes the use of Fourier Transform Infrared Spectroscopy with Total
Attenuated Reflectance (ATR-FTIR), using blood plasma samples from
pregnant women. The study was conducted with women with DMG (n = 50) and
a control group (n = 50) treated at the Department of Gynecology and
Obstetrics at Tertiary Hospital. The blood plasma was inserted in the ATR-FTIR
for analysis of the fingerprint in the region of biological interest (1,800 - 900 cm-
1), analyzed according to the unsupervised models PCA and supervised SPA
and GA, together with the LDA, QDA algorithms, SVM. In general, the results
obtained by GA-LDA were the most satisfactory, using only chosen spectral
variables that could be related to chemical groups of different biomolecules. The
GA-LDA models correctly classified the DMG group vs the Control group, with a
sensitivity and specificity of 96.7% and attributed the main discriminators
between the classes of lipid, carbohydrate and protein biomolecules (from 1,500
to 1,800 cm-1). This study demonstrates the potential of ATR-FTIR techniques
and multivariate models as possible methodologies for the characterization of
GDM. We suggest that the increase in these peaks in the region of protein
biomolecules may be related to the pathophysiology of DMG. Regarding the
outcomes, it was observed that neonates who presented macrosomy were
associated with women with GDM, who had lower density of proteins in the
regions 1,587 cm-1 and 1,586 cm-1. The combination of patient-specific
information with blood biomarker data opens a novel approach to investigate
disease mechanisms in DMG. The approach described here may prove
generally useful for the identification and exploration of DMG under various
physiological and pathophysiological conditions.
Keywords: 

BANKING MEMBERS:
Presidente - 3313589 - JANAINA CRISTIANA DE OLIVEIRA CRISPIM FREITAS
Externa ao Programa - 1755394 - JULIANA FERNANDES DOS SANTOS DAMETTO
Externa ao Programa - 1055045 - MARCELA ABBOTT GALVAO URURAHY
Externa ao Programa - 2149416 - MARIA DA CONCEICAO DE MESQUITA CORNETTA
Externo à Instituição - JOÃO ALEXANDRE TRES PANCOTO - UFES
Externa à Instituição - DIANE MEYRE RASSI - USP