Banca de DEFESA: ANDERSON RODRIGUES DE OLIVEIRA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : ANDERSON RODRIGUES DE OLIVEIRA
DATE: 30/07/2022
TIME: 14:00
LOCAL: remoto
TITLE:

Evaluation of electromyographic, morphological and functional parameters of sciatic nerve regeneration in diabetic animals undergoing hyperbaric oxygen therapy treatment

KEY WORDS:

Diabetes Mellitus. Diabetic Neuropathies. Hyperbaric Oxygenation.

PAGES: 76
BIG AREA: Ciências da Saúde
AREA: Fisioterapia e Terapia Ocupacional
SUMMARY:

Diabetes Mellitus (DM) is a complex disease that requires continuous medical care to reduce risk factors, in addition to glycemic control. Among the main complications of DM, micro and macrovascular disorders related to the renal, cardiovascular and nervous systems stand out. Hyperbaric oxygen therapy (THO) involves intermittent administration of 100% O2 under high pressure, which could reduce damage from hypoxemia and tissue hypoxia in nerve injuries. Objective: To analyze the effects of OTH on sciatic nerve regeneration in diabetic rats. Methods: 49 male Wistar rats, 60 days old, weighing about 220-300g, were divided into four groups: C (normoglycemic animals), C+L (normoglycemic animals submitted to compressive neural injury), DM+ L (diabetic animals submitted to compressive neural injury), DM+L+OTH (diabetic animals submitted to compressive neural injury and OTH). Initially, the animals of the diabetic groups were submitted to DM induction by Streptoozotocin (STZ) and the animals of the groups with lesion were submitted to the induction of sciatic nerve lesion. For the treatment protocol, the animals were submitted to OTH with pressure inside the chamber of 3 absolute atmospheres (ATA) at a rate of 2 ATA/min and will be maintained at this pressure for 60 min. The chamber was ventilated with 100% O2 at a flow of 20 L/min to minimize CO2 buildup. Morphological variables were evaluated using histology, electromyographic (peak amplitude, pulse duration and conduction velocity) and functional variables using the sciatic function index (SFI). Statistical analysis was performed using an analysis of variance (ANOVA), Tukey's test, Kruskal Wallis and Dunn's post hoc test. Results: the animals in the DM+L and DM+L+OTH groups showed a significant increase in glucose levels compared to the control groups (p<0.001) and at the end of the experiment a significant reduction in body weight was observed in the diabetic groups in relation to the control group (p<0.001). There was a significant increase in the number of axons in the GD+L group compared to the GC (p<0.05) and GC+L (p<0.05) groups. Regarding the electrophysiological variables, the GDL+L+OTH group showed significant improvement when compared to the GC (p<0.01) and GD+L (p<0.01) groups. In the assessment of functionality through the SFI, the GD+L+OTH group showed a significant improvement when compared to the GD+L group (p<0.001). Conclusion: OTH promoted an improvement in functional performance and in the amplitude of the electrophysiological signal in diabetic rats submitted to sciatic nerve injury. 

COMMITTEE MEMBERS:
Externo à Instituição - FABRICIO LIMA BRASIL - IINN
Externo à Instituição - FERNANDO AUGUSTO LAVEZZO DIAS - UFPR
Externo à Instituição - FLAVIO SANTOS DA SILVA - UFERSA
Externa ao Programa - 2477216 - NAISANDRA BEZERRA DA SILVA FARIAS - nullPresidente - 2566849 - WOUBER HÉRICKSON DE BRITO VIEIRA