Banca de QUALIFICAÇÃO: SARAH SOPHIA GUEDES LINHARES

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : SARAH SOPHIA GUEDES LINHARES
DATE: 23/11/2022
TIME: 14:30
LOCAL: Centro de Biociências
TITLE:

CONSEQUENCES OF PERINATAL FLUOXETINE TREATMENT ON THE CIRCADIAN ACTIVITY-REST RHYTHM AND ON TIMING SYSTEM COMPONENTS IN MALE AND FEMALE WISTAR RATS.

KEY WORDS:

Fluoxetine; Biological Rhythms; Rats; Timing System; Perinatal Treatment

PAGES: 30
BIG AREA: Ciências Humanas
AREA: Psicologia
SUBÁREA: Psicologia Fisiológica
SPECIALTY: Psicobiologia
SUMMARY:

High perinatal plasticity allows the brain to adapt to its environment, but it also marks a period of heightened vulnerability. The regulation of serotonin levels during development is extremely important for characteristic brain development. Changes in serotonergic signaling, both at low and high levels, appear to be harmful, leading to diverse behavioral deficits. Early exposure to antidepressants, such as SSRIs, during pregnancy or lactation implies several deleterious effects on the anatomical and functional organization in different brain circuits, including those related to the circadian rhythm. In recent years, different authors have investigated these deleterious effects and the mechanisms by which they lead to structural and functional disorganization in specific neural networks, as well as altered behavioral expression in the individual. However, there is a dearth of work at the neurochemical level on structures of the circadian timing system. In this context, this work aims to investigate whether early hyperstimulation of the serotonergic system, through perinatal exposure to an SSRI, can affect the circadian rhythm of activity and rest, especially investigating the expression of neuropeptides and clock proteins associated with the circadian rhythm at different stages of life. of the offspring (PN15, PN45 and PN90). In this study, the locomotor activity-rest rhythm and the visualization of chemical oscillatory markers of the circadian system (NPY, 5-HT, BMAL1, VIP and AVP) will be tested in two zeitgebers (ZT3 and ZT14) in exposed male and female rats. early (developmental period GD13-GD21) to fluoxetine (20 mg/kg). First, with the offspring in adulthood, we performed daily records of the locomotor activity-rest rhythm using activity sensors coupled to individual cages over four weeks, two in light-dark and two in constant-dark. Next, we will identify the expression of neurochemical markers in the suprachiasmatic nucleus, in the intergeniculate leaflet (FIG) and in the raphe nuclei in three life stages (PN15, PN45 and PN90). We hope that, at the end of all the steps, data will be generated that elucidate whether the use of SSRIs during the gestational period causes changes in the serotonergic circuitry in these mentioned regions, in such a way that it causes changes in the behavior of the circadian rhythm.

COMMITTEE MEMBERS:
Interna - 1645202 - ELAINE CRISTINA GAVIOLI
Interno - 2351800 - JEFERSON DE SOUZA CAVALCANTE
Interno - 2998660 - MARIO ANDRE LEOCADIO MIGUEL