Competition is raised as a determinant factor for the organization of local ant community. Conversely, intraspecific competition is an occurrence with important ecological and evolutionary consequences, but its effect in natural populations remains uncertain. The goal of this research was to verify the intraspecific competition between colonies of the Dinoponera quadriceps in their natural habitat. Ten neighboring colonies of D. quadriceps were chosen within the secondary Atlantic Forest area in the Floresta Nacional de Nísia Floresta. The observations took place between July 2019 and February 2020. We observed one ant at a time, from the moment it left its nest until its return. Food collecting, agonistic interaction and chemical marking behaviors were registered using the All-occurrences sampling method. The displacement and places where these behaviors occurred were marked with numbered flags then measured with compass and tape. Results indicate that the colonies of D. quadriceps have foraging areas that do not form territories. In these areas, food collecting occurs with a higher frequency in overlap areas. The workers perform chemical marking unrelated to overlap areas between neighboring colonies. Agonistic interactions had a low frequency of observation, and were characterized by ritualized combat and were more frequent inside overlap areas. Abiotic factors (temperature, humidity, luminosity, rainfall) and biotic (food availability) had no direct influence on the conflicts between colonies.