The formation of new memories is one of the humans and others animals fundamental cognitive capacities. Pattern separation is one of the involved processes in episodic memories encoding, which can reduce interference between similar sensory information and as a effect may facilitate the formation of a new memory that represents the environment in a more refined way. Previous studies revealed that neural oscillations, like theta and gamma, are associated with the encoding of mnemonic informations in the hippocampus, one of the main encephalic regions related with episodic memory formation. However, the relationship of these oscillations to the pattern separation processing in the hippocampus is not well understood in the literature. Therefore, in this project we focus on the process of spatial pattern separation using the object recognition paradigm to look into its repercussions on the neural rhythms of the hippocampus through behavioral and electrophysiological investigations. We evaluated the involvement of theta and gamma neural oscillations in the spatial novelty discrimination of displaced objects in two conditions called high and low interference. To this end, we standardized a new behavioral protocol and simultaneously recorded CA1, CA3 and dentate gyrus local field potential (LFP) of adult male Wistar rats. As a result, we saw that (1) animals showed a preference for exploring the displaced object in both test conditions, and that (2) discrimination index was higher for low than high interference conditions, that is to say, when the object is more displaced from its original position. As a result of the electrophysiological investigation, we observed that (1) there was no significant difference in the theta band (6-10 Hz), and that (2) there was an increase in gamma power in CA1 and CA3 hippocampal areas depending on the object to be explored. In the comparison between displaced objects in BI and AI, CA3 presented greater low gamma power (35-55 Hz) during exploration of the object most displaced from its original position (i.e., BI condition). When comparing displaced and stationary objects in each one of the tests, we found greater low and high gamma power in CA1 (65-90 Hz) during the exploration of the stationary object. Our results suggest that gamma power in CA1 is related to memory recall of objects previously found in their original positions, while the low gamma power in CA3 is related to encoding of new object’s spatial locations. These findings provide an overview of the neural processing dynamics underlying task resolution, and indicate that different areas of hippocampal circuitry are differentially involved in spatial pattern separation.