Banca de DEFESA: LUCIDIO CLEBESON DE OLIVEIRA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : LUCIDIO CLEBESON DE OLIVEIRA
DATA : 07/06/2019
HORA: 14:00
LOCAL: Sala Sagui
TÍTULO:

Analysis of the inoculation of mesenchymal stem cells in the presence of fibroblast growth factor 2 in the morphological and functional regeneration in Facial Nerve Fraction of Wistar rats

PALAVRAS-CHAVES:

FGF-2, Wistar rats, regeneration, stem cells, facial nerve.

PÁGINAS: 75
GRANDE ÁREA: Ciências Humanas
ÁREA: Psicologia
RESUMO:

Studies have shown the influence of the environment on the growth of peripheral nervous system (PNS) injured nerve fibers, as well as the potential for stem cell (CT) use, associated or not with Fibroblast Growth Factor - 2 (FGF - 2), in make this environment more conducive to nerve regeneration. Thus, the study proposes to analyze the effect of the inoculation of mesenchymal CTs in combination or not with FGF-2, in promoting facial nerve regeneration. Wistar rats were submitted to facial nerve crushing in the vicinity of the post-auricular region and were treated or not with CT associated or not with FGF-2. Then, the behavioral evaluation of the animals was performed for 90 days, after which the facial nerve tissue analysis was performed through immunohistochemistry for the growth-associated protein 43 (GAP-43), for the nuclear neuronal protein (NeuN), for Protein glial fibrillary acid (GFAP) and for OX-42. The study showed the influence of the Stem Cells and FGF-2 on the behavior of the motor function of animals submitted to facial nerve injury, evidenced by the behavioral analysis. When comparing the four groups in the histological evaluation, these presented significant differences between them, when comparing the results, it was observed that the groups with CT administration and the CT group associated with FGF-2 and the FGF-2 group presented better results, considering that the Stem Cells groups associated with FGF-2 and FGF-2 groups showed a greater number of fibers, demonstrating a greater axonal budding, thus confirming the properties of FGF-2 in optimizing the differentiation of Stem Cells, as well as stimulate the regenerative process. In view of the results, it is evident that the use of stem cells and FGF-2 potentiated the regenerative process of the facial nerve, providing a more significant functional and histological recovery.

MEMBROS DA BANCA:
Externo ao Programa - 3050287 - EUDES EULER DE SOUZA LUCENA
Interno - 4363493 - EXPEDITO SILVA DO NASCIMENTO JUNIOR
Presidente - 2351800 - JEFERSON DE SOUZA CAVALCANTE
Externo à Instituição - JOSÉ RODOLFO LOPES DE PAIVA CAVALCANTI - UERN
Externo à Instituição - MARCO AURÉLIO DE MOURA FREIRE - FACENE