Banca de QUALIFICAÇÃO: SEBASTIAO PACHECO DUQUE NETO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : SEBASTIAO PACHECO DUQUE NETO
DATA : 27/07/2018
HORA: 08:00
LOCAL: Sala de Aula do PPg em Psicobiologia
TÍTULO:

 

Cytoarchitectonic and neurochemical characterization of the subparaventricular zone (SPVZ) of the common marmoset (Callithrix jacchus)

PALAVRAS-CHAVES:

 

Circadian timing system; Subparaventricular zone; Retinohypothalamic tract; Citoarhitecture; Callithrix jacchus.

PÁGINAS: 94
GRANDE ÁREA: Ciências Humanas
ÁREA: Psicologia
RESUMO:

The circadian timing system (CTS) is composed of a set of neural structures responsible for the generation and modulation of the circadian rhythms of living beings. The suprachiasmatic nucleus (SCN) of the hypothalamus is the structure that generates the circadian rhythmicity, with the 24h environmental light / dark cycle (LD) being its main synchronizer. The SCN generates the expression of circadian rhythms in various diencephalic, telencephalic and forebrain neural structures. Recently, the concept of "expanded CTS" has been proposed, considering that there are approximately 100 neural structures that could compose CTS. Of the different regions involved in this context, the subparaventricular zone (SPVZ), the main efferent site of the SCN, has been highlighted, although it has not yet been studied, especially in non-human primate species such as the Callithrix jacchus, the marmoset. The aim of our study is to describe, through the Nissl method and immunohistochemical techniques, the cytoarchitecture, neurochemical component and retinal projection of the SPVZ of marmosets. From our results we were able to identify the SPVZ cytoarchitecture in this species through the Nissl method and immunoreactivity for the specific neuronal nuclear protein (NeuN). Our data show the SPVZ located between the dorsal and caudal portions of the NSQ, from its middle level and retrochiasmatic region, and ventral portions of the paraventricular hypothalamic nucleus (PHV), distributing lateral to the third ventricle, occupying periventricular portions. The neurochemical content of calcium binding proteins showed an abundance of immunoreactive neurons for calbindin (CB), few immunoreactive neurons for calretinin (CR), but did not detect immunoreactivity for parvalbumin (PV). The CB immunoreactive neurons present a dorsolateral path towards the dorsomedial hypothalamic nucleus (DMH), the main efferent of SPVZ. It was also possible to observe in the ventral and dorsal portions of SPVZ immunoreactive neural fibers to the neuropeptide Y (NPY) and the enzyme glutamic acid decarboxylase (GAD), in the ventral portions we observed fibers immunoreactive to the substance P (SP), whereas in the dorsal portions we observed immunoreactive cells to 5-HT, nitric oxide synthase (NOS), glutamate receptor subunit 1 (GluR1) as well as glial fibrillary acidic protein (GFAP). We also observed a bilateral distribution of immunoreactive retinohypothalamic tract axon terminals (RHT) to the b subunit of cholera toxin (CTb), which demonstrates direct retinal projection for this region.

MEMBROS DA BANCA:
Presidente - 2351800 - JEFERSON DE SOUZA CAVALCANTE
Interno - 2140860 - ROVENA CLARA GALVAO JANUARIO ENGELBERTH
Externo à Instituição - JOSÉ RODOLFO LOPES DE PAIVA CAVALCANTI - UERN