Banca de QUALIFICAÇÃO: INGRID BRASILINO MONTENEGRO BENTO DE SOUZA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : INGRID BRASILINO MONTENEGRO BENTO DE SOUZA
DATA : 20/06/2017
HORA: 09:00
LOCAL: Anfiteatro das Aves
TÍTULO:

Behavioral and neurochemical effects of a NOP receptor antagonist in the dexamethasone-induced depression

PALAVRAS-CHAVES:

Dexamethasone, Glucocorticoid receptors, Major Depression, Animal model, Nociceptin /Orphanin FQ.

PÁGINAS: 101
GRANDE ÁREA: Ciências Humanas
ÁREA: Psicologia
RESUMO:

Major depression can be triggered by stressful events which deregulate the hypothalamic-pituitary-adrenal axis response, promoting, in some circumstances, sustained elevation of circulating glucocorticoid levels. Hypersecretion of glucocorticoids may alter the functionality of glucocorticoid (rG) receptors at the CNS level, especially on the HPA axis and the hippocampus, generating behavioral abnormalities observed in patients with depression. Clinical and preclinical findings suggest antidepressant effects due to the blockade of the NOP receptor signalling. This study aimed to investigate the behavioral and neurochemical effects of the NOP receptor antagonist, SB-612111, on the model of experimental depression induced by acute and repeated administration of Dexamethasone in mice. For this, male and female Swiss mice will be used. The depressive-like behavior will be evaluated through the Tail Suspension Test (TSC), the Splash Test and the Social Interaction Test. In addition, the spontaneous locomotor activity of the mice will be evaluated in the open field. Preliminary findings showed that dexamethasone (64 μg/kg, sc) induced a depressive- like state in males and females by increasing TSC immobility time, self-cleaning latency on Splash and by reducing social interactions. Treatment with the antidepressants Nortriptyline and Venlafaxine reversed most of these effects, with the exception of the social interactions. Importantly, dexamethasone did not affect the spontaneous locomotion of mice, but acute Nortriptyline and Venlafaxine significantly reduced the distance traveled. The treatment with SB-612111, a NOP receptor antagonist, reversed the depressive-like behavior of dexamethasone in the TSC. The effects of the NOP antagonist on dexamethasone-induced depression are in progress in our lab. The present study will further evaluate the effects of repeated administration of Dexamethasone and the antidepressants Nortriptyline and Venlafaxine, as well as SB-612111. Indeed, the oxidative damage to neural cells as well as neurochemical parameters related to apoptosis and neurogenesis (BDNF, Bcl-2 and BrdU) possibly altered by the administration of Dexamethasone will be evaluated during the PhD. In conclusion, this data suggests that acute dexamethasone induces depressive-like effects which are reversed by conventional antidepressants and NOP antagonist. Ultimately, these data provide evidence of the therapeutic potential of NOP antagonists in the treatment of depression.

MEMBROS DA BANCA:
Presidente - 1645202 - ELAINE CRISTINA GAVIOLI
Interno - 6346130 - MARIA BERNARDETE CORDEIRO DE SOUSA
Interno - 2140860 - ROVENA CLARA GALVAO JANUARIO ENGELBERTH