Banca de QUALIFICAÇÃO: VICTOR ANASTÁCIO DUARTE HOLANDA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : VICTOR ANASTÁCIO DUARTE HOLANDA
DATA : 26/04/2017
HORA: 09:00
LOCAL: Sala de Aula da Pós-Graduação em Psicobiologia
TÍTULO:

Role of the nociceptin/orphanin FQ peptidergic system in the mouse depressive-like behavior

PALAVRAS-CHAVES:

Nociceptin/orphanin FQ; antidepressant drugs; NOP receptor; forced swimming test; learned helplessness; mouse.

PÁGINAS: 30
GRANDE ÁREA: Ciências Humanas
ÁREA: Psicologia
RESUMO:

Introduction: Nociceptin/orphanin FQ (N/OFQ) is the endogenous ligand of a Gi protein-coupled receptor, named NOP. Both the N/OFQ and the NOP receptor are expressed widely in brain areas involved in the control of emotional processes. Clinical and preclinical studies support antidepressant effects due to the blockade of NOP receptor signaling. The present study aims to investigate the role of the N/OFQ system in the modulation of depressive-related behaviors in mice. Methods: Male Swiss mice and knockout mice for the NOP receptor (NOP^(-/-)) and their controls (NOP^(+/+)) will be employed in this study. The forced swimming test (FST) and the learned helplessness model (LH) will be used to evaluate the depressive-like behavior of mice. In the first step of this study, the effects of the co-administration of NOP agonists and antidepressant drugs will be assessed in the FST and LH. In the second step, the ability of NOP agonists and antagonists to modulate the acquisition of the helpless phenotype as well as to alter the neurochemical patterns (BDNF, 5-HT, NA and oxidative damage levels) will be evaluated. Indeed, the behavioral phenotype and the neurochemical patterns of NOP^(-/-) and NOP^(+/+) mice exposed to LH will be evaluated. Results: Until now, the behavioral effects of the co-administration of NOP agonists and antidepressants in the TNF has been evaluated. Fluoxetine, nortriptyline, ketamine, UFP-101, and SB-612111 displayed antidepressant-like effects in the FST. The administration of NOP agonists, N/OFQ and Ro65-6570, did not induce any behavioral change, but blocked the antidepressant effects of the NOP antagonists, UFP-101 and SB-612111, and the antidepressants drugs, fluoxetine and nortriptyline, but not ketamine. The effects of the co-administration of antidepressant drugs and NOP agonists on the mouse behavior in the LH remains to be evaluated. In the second step of this study, it is expected that NOP agonists will potentiate the induction of helpless behavior, while NOP antagonists will prevent the acquisition of this phenotype. Furthermore, it is expected that pharmacological manipulations will alter the neurochemical patterns expressed by the animals exposed to LH. Finally, NOP^(-/-) mice are expected to display antidepressant-like phenotype when exposed to LH. Conclusions: Preliminary results suggest that stressufull situations, such as forced swimming, increase the N/OFQergic signaling, inducing a hypomonoaminergic state that could contribute to a pro-depressive profile.

MEMBROS DA BANCA:
Presidente - 1645202 - ELAINE CRISTINA GAVIOLI
Externo ao Programa - 2326928 - JANINE INEZ ROSSATO
Externo à Instituição - LAILA DA SILVA ASTH FERNANDES - UFRN