Banca de DEFESA: JULIA JENSEN DIDONET

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : JULIA JENSEN DIDONET
DATA : 15/03/2017
HORA: 08:30
LOCAL: Anfiteatro das Aves
TÍTULO:

Evaluation of biochemical parameters in two animal models os depression induced by learned helplessness and LPS administration.

PALAVRAS-CHAVES:

Major Depressive Disorder, oxidative damage, learned helplessness, LPS, IL-6, IDO. 

PÁGINAS: 101
GRANDE ÁREA: Ciências Humanas
ÁREA: Psicologia
RESUMO:

Major depression has a great impact on an individual’s quality of life and it is

considered the leading cause of burden in terms of years lost due to disability. However,

despite the severity of depression, the pathophysiology of the disease is still elusive. In

this regard, the use of animal models plays an important role in research for the etiology

of depression. This work compared biochemical alterations occurring on serum, pre-
frontal cortex (PFC) and hippocampus in two animal models of depression: learned

helplessness and administration of lipopolyssaccharide from E.Coli (LPS). Learned

helplessness protocol used in this work resulted in 70 % of helpless mice, assessed by

the inability to escape from electroshocks given 24 h or 48 h after the helpless-induction

session. The other 30 % of mice were considered resilient. Helpless animals showed

more oxidative damage in PFC and serum when compared to controls. No difference

was seen between helpless and resilient groups, but there was a positive correlation

between the oxidative damage on serum and PFC and helpless behavior. There was no

difference in the concentration of IL-1β, TNFα, IL-6 and IL-10 cytokines on PFC and

hippocampus of the animals exposed to the learned helplessness test, but there was a

significant positive correlation between IL-6 concentration and depressive-like behavior

on hippocampus. Indoleamine 2,3-dioxygenase (IDO) enzyme activity was not altered

on learned helplessness model. Systemic administration of LPS (0,8 mg/kg) induced

sickness behavior on animals characterized by decreased food and water intake, body-
weight loss and altered body temperature 6 h after administration. Sickness behavior is

over after 24 h, but LPS-treated mice displayed higher immobility time in the tail

suspension test when compared to saline. There was more oxidative damage in serum,

PFC and hippocampus of LPS group when compared to saline and controls. No

differences on IL-1β and TNFα concentration on serum, PFC and hippocampus of the

animals were detected, suggesting a transient nature of the LPS-induced inflammation.

LPS-treated group displayed higher concentrations of IL-6 on PFC when compared to

saline group, and IL-6 concentration positively correlated to depressive-like behavior.

IL-10 concentrations on hippocampus were negatively correlated to depressive-like

behavior and IDO activity was increased on PFC and decreased on hippocampus of

LPS-treated mice. Data presented here corroborate for the hypothesis of immune

activation during depressive episodes, then resulting in oxidative damage assessed in

two animal models of depression. IDO activity behaved with some specificity in each

animal model depending on the brain or systemic area.

MEMBROS DA BANCA:
Externo ao Programa - 2121895 - ALIANDA MAIRA CORNELIO DA SILVA
Interno - 1696755 - BRUNO LOBAO SOARES
Externo à Instituição - DANIELLE MACEDO GASPAR - UFC
Presidente - 1645202 - ELAINE CRISTINA GAVIOLI
Externo à Instituição - REGINA HELENA DA SILVA - UNIFESP