Banca de DEFESA: YWLLIANE DA SILVA RODRIGUES MEURER

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : YWLLIANE DA SILVA RODRIGUES MEURER
DATA : 13/03/2017
HORA: 08:30
LOCAL: Anfiteatro das Aves
TÍTULO:

Behavioural outcome and neurochemistry analysis of female and male Wistar rats early exposed to fluoxetine

PALAVRAS-CHAVES:

Fluoxetine; early-life brain development; serotonin; inhibitory interneurons.

PÁGINAS: 132
GRANDE ÁREA: Ciências Humanas
ÁREA: Psicologia
RESUMO:

Selective serotonin reuptake inhibitors (SSRIs) are widely prescribed to treat depression, anxiety and other disorders. The developmental exposure to selective serotonin reuptake inhibitors (SSRIs) results in persistent behavioural impairment into adulthood. In this way, serotonergic overexpression in early life may lead to structural and functional changes in brain circuits that control cognitive and emotional behaviour. Here, we addressed the question of how postnatal (PN7-PN21) exposure to fluoxetine affects memory, anxiety- and depression- like behaviours, as well as neurochemical markers of interneurons and serotonergic cells in brain areas related to these behaviours in juvenile (PN45) and adult (PN90) female and male rats. In a first stage, we analysed both female and male rat’s performances in several behavioural tasks and investigated the expression of serotonin (5-HT) in the dorsal and median nucleus of raphe, and parvalbumin (PV) in PFCm and hippocampus at PN45. We found that early-life exposure to fluoxetine increased anxiety- and depression-like behaviours (more in female compared to male animals), and induced a working memory impairment only in the juvenile male. Afterwards, we performed behavioural and neurochemical analysis of male and female adult rats (PND90), where we found that fluoxetine affects only anxiety-related male behaviour. Also, the memory impairment (more in male than female) and depressive-like profile (both sexes) remained in adult age. Moreover, the exposure to fluoxetine affect PV immunoreactivity in the hippocampus in any sex at PN45 and PN90; however, adult animals appear to recover neurochemical deficits observed at the juvenile age. The results revealed developmental fluoxetine effects on juvenile behaviour that can have implications for affective disorders and mnemonic processes. These results revealed persistent changes a sex and age-manner related to developmental exposure to fluoxetine, where serotonergic modulation induce differential profile of anxiety-and depression-like behaviour and mnemonic impairment on female and male rats at juvenile and adult age. Also, suggest a sex-dependent compensatory mechanism, which it is possibly related to serotonergic sinalisation. Circuits may involve sub-cortical and cortical information processing, including subcortical limbic and possible (pre)frontal areas. Thus, our findings suggest that serotonergic modulation during critical periods of SNC development may alter the organisation of excitatory-inhibitory circuit and induce behavioural changes, which may have repercussions for the onset of neuropsychiatric disorders.

MEMBROS DA BANCA:
Presidente - 187.472.708-29 - REGINA HELENA DA SILVA - UNIFESP
Interno - 1645202 - ELAINE CRISTINA GAVIOLI
Interno - 2140860 - ROVENA CLARA GALVAO JANUARIO ENGELBERTH
Externo à Instituição - ALESSANDRA MUSSI RIBEIRO - UNIFESP
Externo à Instituição - DEBORAH SUCHECKI - UNIFESP