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Banca de DEFESA: BEATRIZ KETLYN DA CUNHA BATISTA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : BEATRIZ KETLYN DA CUNHA BATISTA
DATE: 18/03/2024
TIME: 14:00
LOCAL: Link de acesso para videoconferência: https://meet.google.com/kfn-naxb-zju
TITLE: OBTAIMENT OF NANOEMULSION CONTAINING EXTRACT OF THE VEGETABLE SPECIES Jatropha gossypiifolia AND EVALUATION OF ANTIDERMONECROTIC ACTIVITY AGAINST Bothrops erythromelas VENOM

KEY WORDS:

Wounds; Medicinal plants; Antioxidant; Anti-inflammatory; Bothropic envenomation.

PAGES: 113
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Snakebite is a Neglected Tropical Disease of category A, which affects around 2.7

million people per year worldwide. In Brazil, the genus Bothrops has the greatest clinical

relevance, with the species Bothrops erythromelas standing out in the Northeast region. Tissue

necrosis is one of the most relevant local damages caused by this genus, given the possibility

of loss of function of the bitten limb and even amputation, impacting the victim's quality of life.

The ineffectiveness of antivenom in combating local damage caused by envenomation

encourages the search for new complementary therapies, especially medicinal plants. The

species Jatropha gossypiifolia (Jg) is used in popular culture for inflammation, wounds and

snakebites. Therefore, this study aimed to develop a topical nanoemulsion containing the

hydroethanolic extract of Jg leaves, to evaluate the healing and antidermonecrotic activity. The

phytochemical characterization of the Jg extract was performed using High Performance Liquid

Chromatography coupled to a photodiode array detector (HPLC-DAD), which detected the

presence of the flavonoids vitexin, luteolin derivates and apigenin derivatives, and

quantification of the phenolic content and flavonoids, with 95.08 ± 5.46 mg of gallic acid/g of

extract and 137.92 ± 0.99 mg of quercetin/g of extract, respectively. Next, a nanoemulsion

containing Jg extract (NJg) was developed and physicochemical characterization was

performed, in which NJg (n=2) presented an average size of 164.96 ± 9.487 nm, PDI of 0.217

± 0.026, pH of 5.89 ± 0.0281 and phenolic content of 32.039 ± 14.628 mg of gallic acid/g of

extract, showing no color variation or phase separation. Over the course of 4 weeks, the

characteristics were maintained. To evaluate in vitro cytotoxicity, the hemolytic and crystal

violet assay was performed, in which the extract and NJg did not show toxicity at the

concentrations tested (32.25-250 µg/mL) for human erythrocytes and murine fibroblasts

(NHI/3T3). Furthermore, the antioxidant activity of the extract and NJg was performed,

reaching an IC50 of 0.2999 and 0.4646 µg/µL for the ABTS∙+ radical, respectively, and of

0.2899 and 0.4374 µg/µL for the radical DPPH, respectively. Next, the skin irritation of the

NJg was evaluated, demonstrating the absence of irritation. In the evaluation of healing activity

in vivo, NJg presented significant wound closure from the 5th day of treatment, when compared

to the no treatment group, being 45% for NJg and 21% for the no treatment group, and on the

8 th day reaching 58%. Besides, NJg promoted the reduction of nitrite, myeloperoxidase activity

and cytokines. An evaluation of the in vitro inhibition of phospholipase A2 (PLA2) and

hyaluronidase from Bothrops erythromelas venom (BeV) was also carried out, with maximum

inhibition of 23% (1:100; BeV:NJg) and 24% (1:0.25; BeV:NJg), respectively. Finally, the in

vivo inhibition of the dermonecrotic halo caused by Pbe was evaluated, demonstrating that NJg

was able to reduce the formation of this halo from 24h after induction. Therefore, it can be

suggested the participation of the extract's compounds in the pharmacological action, especially

the flavonoids, which mainly have antioxidant and anti-inflammatory actions. In conclusion,

the results suggest that the developed NJg is promising and may be contributing to the treatment

of local damage from snakebite.

COMMITTEE MEMBERS:
Externo à Instituição - ANGELO RONCALLI ALVES E SILVA - UNIFOR
Externa à Instituição - JACINTHIA BEATRIZ XAVIER DOS SANTOS - UNINASSAU
Externa à Instituição - MAIRA CONCEIÇÃO JERONIMO DE SOUZA LIMA - UnP
Presidente - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA

Notícia cadastrada em: 28/02/2024 19:48