Banca de QUALIFICAÇÃO: JOAO FELIPE OLIVEIRA DA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : JOAO FELIPE OLIVEIRA DA SILVA
DATE: 19/02/2024
TIME: 14:00
LOCAL: Link de acesso para videoconferência: https://meet.google.com/nxu-mhnc-bsp
TITLE:

ANTINOCICEPTIVE AND ANTIEDEMATOGENIC ACTIVITY OF Ipomoea
pes-caprae EXTRACT INCORPORATED IN A GEL AGAINST POISONING BY
Tityus stigmurus

KEY WORDS:

Antiedematogenic. Nociception. Scorpionism. Ipomoea. Tityus.

PAGES: 53
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Accidents involving venomous animals are considered neglected diseases according to
the World Health Organization. In Brazil, the number of accidents involving scorpions
has been growing over the years, with Tityus stigmurus being the main responsible for
accidents in northeastern Brazil. Currently, the treatment for cases of accidents with
scorpions consists of serotherapy through the administration of antiscorpion or
antiarachnid serum. However, serotherapy has low efficacy in inhibiting the effects at
the site of the sting, high production costs and a heterogeneous geographic distribution,
important limitations that drive research into new forms of complementary treatment in
cases of accidents with scorpions. The wide repertoire of bioactive molecules in
different plant species with anti-inflammatory potential indicates the potential beneficial
effect of these components against the effects caused by scorpion poisoning. In this way,
the antiedematogenic and antinociceptive capacity of the hydroethanolic extract of the
leaves of the plant species Ipomoea pes-caprae was evaluated in its free form and
incorporated into gel, in in vivo models of inhibition in mice. The characterization of the
formulation was carried out through observation of macroscopic characteristics, pH
analysis and phenolic dosage. The evaluation of antiedematogenic activity was observed
through the paw edema model induced by T. stigmurus venom. The animals showed a
significant reduction in edema when treated with the extract of I. pes-caprae leaves,
presenting a percentage of edema of 7.98% when treated with the 5% Ipc gel. The
formalin test was used as a model to evaluate nociception using 2.5% formalin as a
nociceptive agent and morphine and indomethacin as a control group. The animals
treated with the 5% Ipc gel showed a significant reduction in the time that the mice
spent licking their paws as an indication of pain, both in the first phase (neurogenic) and
mainly in the second phase (inflammatory), showing behavior similar to indomethacin.
This test also demonstrated the extract's ability to reduce the activity of the
myeloperoxidase enzyme in nociception tests. Thus, the results obtained demonstrate
the potential of Ipomoea pes-caprae extract as an alternative complementary treatment
in cases of scorpionism induced by scorpions of the genus Tityus.

COMMITTEE MEMBERS:
Interna - 2374605 - AURIGENA ANTUNES DE ARAUJO
Presidente - 2378605 - CRISTIANE FERNANDES DE ASSIS
Interna - 1055045 - MARCELA ABBOTT GALVAO URURAHY