Banca de QUALIFICAÇÃO: GILSON CASSIANO DE GÓES NETO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : GILSON CASSIANO DE GÓES NETO
DATE: 10/07/2023
TIME: 13:30
LOCAL: Sessão de videoconferência: http://meet.google.com/kss-xraq-own
TITLE:

Anti-SARS-CoV-2 evaluation in vitro of new release systems with naphtoquinones derivatives

KEY WORDS:

SARS-CoV-2; COVID-19; naphthoquinones; IVS320; cyclodextrins; inclusion complexes; drug delivery systems; pharmaceutical technology.

PAGES: 93
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

The outbreak of COVID-19, a disease transmitted by the SARS-CoV-2 virus, is rapidly developing on a global scale, causing millions of deaths worldwide. With this, it is necessary to develop new compounds that can act in the treatment of COVID-19. Naphthoquinones (NQs) and their derivatives have antifungal, antiparasitic and antimicrobial action, among which is the compound IVS320, a molecule with promising antimicrobial action, but with low solubility. Cyclodextrins (CDs) are used as a strategy to improve the solubility, stability and bioavailability of drugs. The development of inclusion complexes (ICs) with cyclodextrins can overcome the implicit restrictions of IVS320, increasing its bioavailability and improving its pharmacological action. Thus, the present study aims to improve the physicochemical and biological properties of naphthoquinone IVS320, through the development of inclusion complexes. In addition to evaluating the in vitro anti-SARS-Cov-2 activity of the delivery systems and analyzing the bioactive potential of the compound. The complexes obtained with γ-CD, HP-γ-CD and HP-β-CD, through physical mixing (MF), kneading (ML) and rotary evaporation (RT) techniques, were characterized using DRX techniques , FTIR, DSC and TG. The XRD results of the IVS320 alone show crystalline reflections with great intensity at 10°, 18°, 32° and 37°, while the diffractograms of the MF, ML and RT systems show a reduction of the crystalline profile, suggesting the formation of complexes. The FTIR spectra of the systems knew characteristic bands of both the IVS320 and the employed CDs, with modifications in the profile of some bands. In turn, the DSC showed similarity with the endothermic and exothermic events found in the isolated IVS320, the IC exhibited the main occurrences in the ranges of 70-80°C, 190-200°C, up to exothermic peaks in the range of 250°C - 260°C, signaling crystallization. While in TG there was a well-defined mass loss for all complexation techniques, in which the first events of the systems occurred around 50°C to 70°C, with about 10% of mass loss, since the second variation started between 300 to 340°C, followed by a gradual mass decay up to 600°C, with the highest Δm (approximately 80%). Regarding in vitro activity, the compound IVS320 showed a very strong result, being able to inhibit the SARS-CoV-2 virus. The results of the physical-chemical characterization indicated the formation of IVS320 ICs with CDs, showing that the methods employed were effective and that the systems presented become promising to potentiate the antiviral activity of IVS320.

COMMITTEE MEMBERS:
Presidente - 1893445 - EUZEBIO GUIMARAES BARBOSA
Externo ao Programa - 2140814 - FERNANDO HENRIQUE ANDRADE NOGUEIRA - nullExterna à Instituição - THALITA SÉVIA SOARES DE ALMEIDA MAGALHÃES - UniFIC