Banca de DEFESA: LARISSA FERREIRA RIBEIRO DE OLIVEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LARISSA FERREIRA RIBEIRO DE OLIVEIRA
DATE: 30/06/2023
TIME: 14:00
LOCAL: Sessão de videoconferência: https://meet.google.com/xna-tfhe-vko
TITLE:

Simultaneous vehiculation of probiotics and yellow mombin (Spondias mombin L.) seed extract to develop a new vegan multifunctional ingredient

KEY WORDS:

Spondias mombin L.; probiotic; phenolic compounds; microencapsulation; spray-drying.

PAGES: 112
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

The association of probiotics and plant extracts is a promising strategy for the simultaneous delivery of functional compounds, conferring numerous health benefits. In this context, the present study aimed to evaluate the bioactive potential of cajá seeds (Spondias mombin L.) and their simultaneous delivery with probiotics, using fermentation and co-encapsulation as strategies. Initially, the cajá seeds were subjected to physical-chemical characterization and evaluated as a source of phenolic compounds in different extracting solutions. Ethanol 50% favored a higher concentration of phenolics (293.81 ± 8.50 mg GAE/L) and total sugars (21.03 ± 0.34 g/L). Then, this extract was evaluated for the multiplication capacity of Lactobacillus acidophilus NRRL B-4495 and Lactiplantibacillus plantarum NRRL B-4496. During 48h of fernentation, probiotic populations descreased by 1.6 to 2.2 logarithimic cycles and maintained the phenolic content. To increase the stability of the product, L. acidophilus and the cajá seeds extract were co-encapsulated by spray-drying using maltodextrin isolated (EPM) or associated with citrus pectin (EPMP). The extract and microcapsules were evaluated for physicochemical characteristics and antioxidant and antibacterial activities. Furthermore, probiotic survival under simulated gastrointestinal conditions was investigated. The EPM and EPMP microcapsules presented mean diameters of 16.37 ± 5.42 μm and 15.18 ± 2.52 μm and encapsulation efficiency above 98%. EPM showed a higher concentration of total phenolics (121.40 ± 1.22 mg GAE/g) while EPMP showed higher antioxidant activities. Regarding antibacterial activity, the extract did not show inhibition at the tested concentrations, EPMP showed a minimum inhibitory concentration (MIC) of 250 μg/mL for S. aureus and 500 μg/mL for E. coli, while EPM showed a MIC of 250 μg/mL for S. aureus and E. coli. The survival rate of encapsulated under simulated gastrointestinal conditions was higher in gastric fluid. Overall, spray-drying was a viable strategy for the co-encapsulation of probiotics and cajá seeds extract, enhancing their biological activities and contributing to the diversification of probiotic products available on the market.

COMMITTEE MEMBERS:
Interna - 2378605 - CRISTIANE FERNANDES DE ASSIS
Externa à Instituição - ESTER RIBEIRO DE ANDRADE - UFPE
Presidente - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Externa ao Programa - 1517220 - MARCIA REGINA DA SILVA PEDRINI - null