Banca de DEFESA: CHRISTIANE CARLOS ARAÚJO DE NEGREIROS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CHRISTIANE CARLOS ARAÚJO DE NEGREIROS
DATE: 15/06/2023
TIME: 14:00
LOCAL: Link de acesso para videoconferência: https://meet.google.com/zab-uvvw-fqx
TITLE:

 EXPERIMENTAL INFECTION OF TRIATOMA BRASILIENSIS AND TRIATOMA PSEUDOMACULATA (HEMIPTERA: REDUVIIDAE) WITH DIFFERENT TRYPANOSOMA CRUZI GENOTYPES

 

KEY WORDS:

Trypanosoma cruzi. Triatomines. Experimental infection. Biological aspects.

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

This study aimed to evaluate the biological aspects of Triatoma brasiliensis and Triatoma pseudomaculata experimentally infected by different DTUs (Discrete Typing Units) of Trypanosoma cruzi. Third, fourth, and fifth instar nymphs of T. brasiliensis (n=120) and T. pseudomaculata (n=120) were fed on mice infected with one or more T. cruzi isolates. A group of uninfected insects was used as control. Infection of triatomines by T. cruzi was evaluated daily and individually by analysis of excreta up to 90 days after infection. Biological aspects of triatomines such as excretion, occurrence of ecdysis and mortality were evaluated daily. The volume of ingested blood (μL) and the weight (mg) of the insects was verified 4h after the blood meal, which occurred every 15 or 30 days. T. cruzi infection was detected in all groups of T. brasiliensis, whereas in T. pseudomaculata the infection was only observed in the group with single infection by the RN02 isolate and in the groups with mixed infection RN02+RN23 and RN02+RN23+CL-Brener. The average percentage of infection in all groups of T. brasiliensis was 20% and 22%, in insects that performed blood meals at intervals of 15 and 30 days, respectively. For T. pseudomaculata the average percentage of infection was 18% (feeding every 15 days) and 8% (feeding every 30 days). The volume of ingested blood by the groups of T. brasiliensis and T. pseudomaculata with mixed infection was lower when compared to the groups with single infection and the control. The occurrence of ecdysis was higher in the groups of T. brasiliensis with single infection and in the control group of T. pseudomaculata. Mortality was higher in T. pseudomaculata groups with mixed infection, whereas no significant difference was observed in T. brasiliensis groups. Excretion was higher in the T. pseudomaculata control group. The occurrence of kleptohematophagy in T. brasiliensis was described for the first time. These data showed that these two species are potentially effective vectors in the transmission of T. cruzi, with T. brasiliensis being more susceptible to infection. Therefore, carrying out vector control activities continuously and efficiently is essential to reduce the risk of T. cruzi transmission in endemic areas where these vectors occur.

COMMITTEE MEMBERS:
Externa à Instituição - ANDRESSA NORONHA BARBOSA DA SILVA - UFRN
Presidente - ***.985.294-** - LUCIA MARIA DA CUNHA GALVAO - UFRN
Externa ao Programa - 3270997 - NATHALIE DE SENA PEREIRA - nullExterno à Instituição - VAGNER JOSE MENDONCA - UFPI