Banca de DEFESA: ULANA CRISTINA DE ARAUJO TAVARES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ULANA CRISTINA DE ARAUJO TAVARES
DATE: 23/01/2023
TIME: 09:00
LOCAL: Sessão de videoconferência: https://meet.google.com/chk-tagt-zhf
TITLE:

Anti-inflammatory and healing evaluation of chitosan base-films with α, β-amirenone triterpene.

KEY WORDS:

Chitosan films; α, β-Amirenone; Physicochemical characterization, healings; bandages; in vivo studies.

PAGES: 82
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

α, β-amirenone (ABAME) is a natural compound of the triterpene class, which has great potential for anti-inflammatory and healing activity. Chitosan films are increasingly used for topical use because they allow a gradual release of the drug and have many advantages such as biodegradable, flexible, durable and resistant, they do not break or tear easily. Thus, the objective of this study is to develop, characterize and evaluate chitosan films incorporated with α, β-amyrenone, in addition to analyzing the anti-inflammatory and healing effect, aiming at topical application in the treatment of skin lesions. The ABAME systems were evaluated by the following techniques: FTIR, DR-X, DSC, TG and SEM. In the in vivo part, the animal model was mice, which were subjected to injuries on the back and followed for 14 days to assess the healing process of the injury. After euthanasia, the quantification of cytokines, the macroscopy of the lesions and the histological analysis were evaluated. Thermal analysis demonstrated sample incorporation and an increase in the thermal stability of ABAME. The FTIR spectra of the films demonstrated interactions occurring between ABAME and the chitosan matrix. XRD results showed peaks in halos, characteristic of amorphous compounds, indicating the amorphization of ABAME by chitosan. SEM images showed the prototype homogeneously incorporated into the chitosan matrix and with partial loss of the crystalline characteristic of pure ABAME. In vivo, it was possible to observe that therapy with films incorporated with ABAME is quite effective, since it has anti-inflammatory properties that help in the healing process of skin lesions, making it possible to evaluate this evolution macroscopically, histologically and with the cytokines measured in the present study. In this study, the analyzed factors suggest that the dressing of chitosan with ABAME had a regenerating effect in mice, minimizing the parameters of the inflammatory process and progressing healing. It suggests the development of observational studies of the long-term effects of dressings, in addition to other analyzes that can verify that they do not change over time, as well as evaluating their effectiveness in scar tissue.

COMMITTEE MEMBERS:
Presidente - 1789788 - ADLEY ANTONINI NEVES DE LIMA
Externo à Instituição - EMERSON SILVA LIMA - UFAM
Externo à Instituição - JOSÉ LAMARTINE SOARES SOBRINHO - UFPE