Portal de Programas de Pós-Graduação (UFRN)

SIGAA - Sistema Integrado de Gestão de Atividades Acadêmicas

PPGCF/CCS PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS FARMACÊUTICAS ADMINISTRAÇÃO DO CENTRO DE CIÊNCIAS DA SAÚDE Phone: (84) 99193-6161 E-mail: pgfar@ccs.ufrn.br https://posgraduacao.ufrn.br/ppgcf

Banca de DEFESA: KYVIA REGINA FERNANDES VARELA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : KYVIA REGINA FERNANDES VARELA
DATE: 29/11/2022
TIME: 09:00
LOCAL: Link de acesso para videoconferência: meet.google.com/iim-vdyf-dfn
TITLE: DESENVOLVIMENTO DE NANOFORMULAÇÕES DE PROTÓTIPOS DE VACINAS DE DNA E AVALIAÇÃO DE PARÂMETROS FÍSICO-QUÍMICOS E BIOLÓGICOS

KEY WORDS:

Plasmids; DNA vacines; Polymeric nanoparticles; Polyethyleneimine; Gene Transfer; Nucleases.

PAGES: 98
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

DNA vacines, also called genetic vacines, are based on the use of plasmids that encode

pathogen gene sequences as an antigenic candidate for inducing immunity. These vaccines have

numerous advantages when compared to other vaccines; are easy to develop, low cost of

production, safety and induction of cellular and humoral immune response. However, its main

disadvantage is a reduced rate of cell transfection and consequently low endogenous production

of the antigen of interest. This disadvantage is mainly due to the degradation of plasmids by

nucleases present in tissues and inside cells. Thus, this study aims to develop a nanosystem

containing plasmids used in DNA vaccine models. Specifically plasmids pVAX1, pVAX1lacZ

and nTSApVAX1, incorporated into polymeric polylactic acid (PLA) nanoparticles, with and

without the presence of polyethyleneimine (PEI), which can protect and increase the levels of

gene transfer of the plasmids, thus, building efficient vaccine prototypes. Therefore, after

formulation, the physicochemical parameters of the systems were evaluated from the mean

particle diameter, polydispersity index (PDI) and zeta potential. Additionally, performance tests

were performed, such as: physical-chemical stability, electrophoretic mobility delay, protection

against enzymatic degradation on different sources and types of enzymes, and in vitro cell

viability. The nanoformulations produced had an average size around 250 nm, PDI <0.3. The

cationic nanoformulations (PLA + PEI + pDNA) showed good complexation between polymers

and pDNA, as there was no appearance of bands in the electrophoresis assays, even against the

degradation enzymes, also suggesting protection. The same profile was not observed with the

anionic nanoformulations (PLA + pDNA), in which bands referring to the pDNAs appeared,

indicating a not so efficient binding between the componentes. Moreover, all systems showed

good physicalchemical stability during a six weeks period, as well asan adequate cell viability

(>70%), not showing any relevant citotoxicity. Thus, nanoformulated pDNAs are shown to be

promising and safe carriers in the development of DNA vaccine prototypes.

COMMITTEE MEMBERS:
Presidente - 2275890 - MARCELO DE SOUSA DA SILVA
Externa ao Programa - 2085604 - SUSANA MARGARIDA GOMES MOREIRA - nullExterna à Instituição - WENDY MARINA TOSCANO QUEIROZ DE MEDEIROS - IFRN

Notícia cadastrada em: 11/11/2022 17:22