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Banca de QUALIFICAÇÃO: VICENTE TOSCANO DE ARAUJO NETO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : VICENTE TOSCANO DE ARAUJO NETO
DATE: 08/09/2022
TIME: 14:00
LOCAL: Link de acesso para videoconferência: https://meet.google.com/hhp-kead-gfu
TITLE: Fluralaner (Bravecto®) induces long-term mortality of Lutzomyia longipalpis after a blood meal in treated dogs

KEY WORDS:

Trypanosomatids, Trypanosoma cruzi, Leishmania spp, sheep, goats, horses, canids.

PAGES: 101
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Trypanosomiasis are protozoa that occur in the Americas, highlighting the infection by

Trypanosoma cruzi and Leishmania spp. In this study, the occurrence of natural infection by

trypanosomatids in peridomiciled animals was evaluated using molecular markers and

phylogenetic analysis. The samples were blood clots from 163 animals: 60 sheep (Ovis aires),

22 goats (Capra hircus), 14 horses (Equus caballus), three cattle (Bos tauros) and 64 dogs

(Canis domiciliares) and 64 aspirated from popliteal lymph node of dogs in nine municipalities

in the state of Rio Grande do Norte (RN). Popliteal aspirate samples were added in culture and

the isolates were also analysed by molecular markers and phylogenetic analysis. Natural

infection by Trypanosoma spp. was identified by amplification of the 18S SSU rRNA gene in

48.9% of goat, sheep and equine samples. In the phylogenetic analysis, the SH022 sample

showed 99.8% similarity with the Y strain of T. cruzi grouping in the DTU II clade. Clot

samples from sheep, goats and horses amplified T. cruzi kDNA by 28.3% (17/60), 22.7% (5/22)

and 15.4% (2/14), respectively. These samples were distributed in the three studied

mesoregions. Data with dog samples revealed that popliteal cultures in biphasic medium were

12.5% (8/64) of the positive samples with flagellated forms similar to promastigotes, preceded

by the cities of Marcelino Vieira (3/6), Alexandria (3 /3) and Christmas (2/7). Blood clot

samples from dogs were also submitted to T. cruzi kDNA amplification and showed 10.9%

(7/64) positivity. In addition to the clots, the DNA from the wet mass of parasites from the eight

positive samples in the cultures was submitted to the same amplification showing bands of

650bp. In the sequencing and phylogenetic analysis, all samples of the culture showed similarity

above 99% with the Leishmania chagasi sequences. The wide distribution of T.cruzi and

Leishmania chagasi infection in domesticated animals contributes to the expansion of the

epidemiological scenario and the need to better understand the role of these animals in the

transmission cycle as reservoirs and/or sentinels of infections in humans.

COMMITTEE MEMBERS:
Externa ao Programa - 1714243 - DANIELLA REGINA ARANTES MARTINS SALHA - nullExterno ao Programa - 1046091 - JOAO FIRMINO RODRIGUES NETO - nullPresidente - 1055045 - MARCELA ABBOTT GALVAO URURAHY

Notícia cadastrada em: 25/08/2022 11:05