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Banca de QUALIFICAÇÃO: JACINTHIA BEATRIZ XAVIER DOS SANTOS

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : JACINTHIA BEATRIZ XAVIER DOS SANTOS
DATE: 02/06/2022
TIME: 14:00
LOCAL: Link de acesso para videoconferência: https://meet.google.com/woj-ivah-xbk
TITLE:

Efficacy of the gel based on the plant extract of Ipomoea pes-caprae in the inhibition of the local toxicity produced by the venom of the snake Bothrops erythromelas

KEY WORDS:

Ipomoea pes-caprae. Bothropic. Convolvulaceae. Formulation. Antiophidic.

PAGES: 84
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Snakebites is considered a relevant public health problem, included by the World

Health Organization in the list of Neglected Tropical Diseases. The most clinically

relevant genus, responsible for the largest number of accidents, is Bothrops. Common

events such as local edema, myotoxicity, hemorrhage and dermonecrosis characterize

accidents and can lead to severe damage to patients. The low efficiency of antivenom

serum therapy in the face of these events encourages the search for complementary

therapies. Medicinal plants have been used for years in traditional medicine in snakebites.

The plant species Ipomoea pes-caprae (Ipc), popularly known as “salsa-da-praia”, is used

in traditional medicine to treat inflammation, pain, burns and snake bites. Thus, this study

focused on the development and evaluation of formulations containing Ipc extract

regarding their antiophidic potential. The hydroethanolic extract of Ipc was obtained by

maceration. The results suggested by Thin Layer Chromatography and confirmed by the

analysis by High Performance Liquid Chromatography identified the majority presence

of phenolic compounds in the extract. Obtaining the fraction enriched with phenolic

compounds (FrEtOH) was successfully performed. The extract was incorporated into a

gel previously developed by the research group. After 90 days of the accelerated stability

study, the formulations with the extract were stable in terms of organoleptic and

physicochemical properties, as well as the phenolic content with variation according to

the legislation. The Ipc and FrEtOH extracts showed no in vitro cytotoxicity against

murine macrophages (RAW 267.3) and mouse embryonic fibroblasts (NIH/3T3) cells.

The in vitro enzymatic activities against Bothrops erythromelas (Ber) venom toxins

(phospholipase, proteolytic and hyaluronidase) were similarly inhibited by the Ipc extract

and FrEtOH. The in vivo activities against the local effects produced by the Ber venom

(edematogenic, hemorrhagic and dermonecrosis) were reduced by the treatment with the

Ipc gel 5%, as well as a synergistic effect when using the Ipc gel associated with the

antivenom. The inhibitions observed were promising, presenting a direct action of the

extract inhibiting venom toxins and an indirect action through the inhibition of

endogenous mediators involved in envenomation. In addition, it is possible to relate the

activity to the compounds present in the extract, with emphasis on the phenolic

compounds that act strongly as anti-inflammatory, antioxidant and metal chelator. Given

this scenario, the results point to the potential of the developed formulation, which may

justify the medicinal use of the extract in snake bites, and may also contribute to the

development of new herbal products on the market for the treatment of the local effects

of bothropic envenomation.

BANKING MEMBERS:
Presidente - 2330188 - GERLANE COELHO BERNARDO GUERRA
Externa à Instituição - JULIA MORAIS FERNANDES - UPC
Externa ao Programa - 067.469.464-36 - YAMARA ARRUDA SILVA DE MENEZES - UFRN

Notícia cadastrada em: 06/05/2022 11:07