Banca de QUALIFICAÇÃO: STPHANNIE JAMYLA DE ARAÚJO BARBOSA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : STPHANNIE JAMYLA DE ARAÚJO BARBOSA
DATE: 20/05/2022
TIME: 13:30
LOCAL: Link de acesso para videoconferência: https://meet.google.com/uab-sqtx-bjg
TITLE:
Lactobacillus casei prevents inflammation and increases barrier proteins in an experimental model of intestinal mucositis

KEY WORDS:
Mucositis; Probiotics; Lactobacillus

PAGES: 70
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:
The aim of this study was to evaluate the effect of on an intestinal mucositis
model. Lactobacilus casei (1x109 CFU/ml) or saline was orally administered for
18 days. On 15th day, intestinal mucositis was induced by a single intraperitoneal
administration of 5-FU (450 mg/kg). The animals were euthanized and segments
Swiss mice of small intestine were collected to evaluate immunohistochemistry
and histopathological, acetylcholinesterase activity, sucrose activity, concentration
of inflammatory cytokines, oxidative stress markers and genic expression. The L.
Casei group showed a protective effect, maintaining the activity of
acetylcholinesterase enzyme when compared to the 5FU group (p<0.001), even
though they were exposed to 5FU on the 15th day. L. Casei group showed
preserved enzymatic activity, with no significant reduction in relation to the saline
group (p˃0.05). The administration of L. Casei probiotic reduced the levels of
MDA (p < 0.001) and prevented damage to the intestinal mucosa with a significant
reduction in the levels of the inflammatory cytokines IL-1 beta, IL-6, and TNF
alpha ( p ˂ 0.001). Histological results showed Score 1, villus blunting, loss of
crypt architecture, sparse inflammatory cell infiltration, vacuolization, and normal
muscle layer edema. The use of the probiotic was capable to prevent rising NFKB
P65 gene expression when compared to the 5FU group (p<0.05), and significantly
preventing the increase in TLR-4 gene expression when compared to the 5FU
group (p<0.001)

BANKING MEMBERS:
Externa ao Programa - 2578619 - ANA HELONEIDA DE ARAUJO MORAIS
Interno - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Presidente - 2330188 - GERLANE COELHO BERNARDO GUERRA
Notícia cadastrada em: 02/05/2022 11:56
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