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Banca de DEFESA: MIKAELI MEDEIROS DANTAS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MIKAELI MEDEIROS DANTAS
DATE: 23/02/2022
TIME: 14:00
LOCAL: Link de acesso para videoconferência: https://meet.google.com/acf-efnt-doi
TITLE:

EVALUATION OF ANTINOCICEPTIVE ACTIVITY AND SUBCHRONIC TOXICITY OF THE HYDROETHANOLIC EXTRACT OF THE BARK OF THE STEM OF Commiphora leptophloeos

KEY WORDS:

Burseraceae. Imburana. Nociception. Pain. Herbal medicines.

PAGES: 111
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

The plant species Commiphora leptophloeos (Mart.) J.B. Gillett, stands out for being a

medicinal plant used in the treatment of inflammation in general. Therefore, this work aims to

evaluate the antinociceptive activity and subchronic toxicity of the hydroethnolic extract of the

stem bark of C. leptophloeos in mice. Initially, subchronic toxicity was evaluated for 28 days at three

different doses (200, 400 and 800 mg/kg) orally and behavioral analyzes were performed (Hippocratic

screening, Campo Aberto test and Rotarod), mortality, alteration of hematological parameters,

alterations macroscopic and histopathological analysis of organs (heart, liver, spleen and kidneys), and

evaluation of markers of inflammation and oxidative stress (MPO and MDA).The potential

antinociceptive effect of the EHCL (100, 200 and 400 mg/kg) was evaluated in vivo through

models of pain induced by chemical and thermal agents, such as the abdominal writhing tests

induced by acetic acid, formalin and hot plate, respectively. In order to evaluate the participation

of opioid receptors, ATP-sensitive K+ channels and the L-arginine-NO pathway in the

antinociceptive action, pre-treatment with naloxone, glibenclamide or methylene blue was

performed, respectively, before the administration of the dose of 400 mg/kg. The subchronic

toxicity study showed that the oral extract at doses of 200, 400 and 800 mg/kg did not present

significant changes in behavioral, locomotor and hematological parameters. However, on

histological analysis, the dose of 800 mg/kg showed signs that indicate nephrotoxicity. The

EHCL at doses of 100 and 200 mg/kg showed an antinociceptive effect against chemical

stimuli, while the dose of 400 mg/kg showed an antinociceptive effect against chemical and

thermal stimuli. Glibenclamide associated with EHCL partially reduced the antinociceptive

effect of the extract, demonstrating possible involvement with ATP-sensitive K+ channels. The

administration of methylene blue with EHCL caused a potentiation of the antinociceptive effect

of the extract, indicating involvement in the cGMP pathway. The use of naloxone together with

the extract resulted in the inhibition of the analgesic effect of EHCL, demonstrating that the

extract is involved in the opioid system. Finally, the results obtained in this work justify the use

of C. leptophloeos stem bark in folk medicine to treat pain and contribute to the development

of a possible safe and effective herbal medicine with analgesic properties.

BANKING MEMBERS:
Interna - 2374605 - AURIGENA ANTUNES DE ARAUJO
Presidente - 2330188 - GERLANE COELHO BERNARDO GUERRA
Externo à Instituição - JULIANA KÉSSIA BARBOSA SOARES - UFCG

Notícia cadastrada em: 10/02/2022 16:33