Banca de DEFESA: ELAINE CRISTINE SOUZA DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ELAINE CRISTINE SOUZA DA SILVA
DATE: 22/11/2021
TIME: 14:00
LOCAL: Link de acesso para videoconferência: https://meet.google.com/yse-cpoh-xcg
TITLE:

Phytochemical Study and Evaluation of the Gastroprotective Effect of Cactaceae Nopalea cochenillifera

KEY WORDS:

Gastric ulcers. Cytokines. Antioxidant. Flavonoids.

PAGES: 157
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Nopalea cochenillifera, from the Cactaceae family, is popularly known as “sweet” or “small” palm and is widely cultivated in the Northeast region of Brazil. This research aimed to characterize the chromatographic profile and evaluate the gastroprotective effect of the hydroethanolic extract of Nopalea cochenillifera. For this, the cactus was collected, cut and dried in an oven, crushed, macerated with ethanol and water, obtaining the hydroethanolic extract, which was lyophilized. The phytochemical study of the hydroethanolic extract was carried out by Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography Coupled to a Mass Spectrometer (HPLC-ESI-IT). The evaluation by CCD indicated the presence of phenolic compounds, including flavonoids. Analysis by HPLC-ESI-IT identified the presence of six compounds, with a predominance of hexoside caffeic acid. In the pharmacological study, to evaluate the gastroprotective activity, Rattus norvegicus (Wistar) were used, randomly distributed in groups (n=7) control gastric lesions, healthy group (saline), extract of N. cochenillifera (50, 100 and 200 mg/kg ) and ranitidine (50 mg/kg). In the models induced by ethanol and indomethacin, the extract showed that it prevented the formation of gastric lesions, at a dose of 100 mg/kg, ethanol model (P<0.001); and at doses of 100 mg/kg (P<0.05) and 200 mg/kg (P<0.01), in the indomethacin model. The macroscopic evaluation of the stomachs showed a reduction in the ulceration index, accompanied by a reduction in the activity of myeloperoxidation (MPO), and malondialdehyde (MDA), preservation of GSH, reduction in gastric levels of pro-inflammatory cytokines (TNF-α and IL-1β ), and elevation of IL-10. Histopathological analyzes revealed that the extract prevented the morphological changes induced by ethanol and indomethacin, preserving the gastric tissue integrity, and also reduced the inflammatory infiltrate. In immunohistochemical analysis, the extract decreased the expression of the COX-2 enzyme and increased the expression of SOD at a dosage of 200 mg/kg when induced by indomethacin and ethanol. Pre-treatment with N. cochenillifera extract did not change the pH, volume or total acidity of the gastric juice, but preserved the secretion of mucus, which could exert a cytoprotective effect. The results of the present work provide preclinical evidence that the extract of N. cochenillifera has gastroprotective activity, which may be related to the richness of phenolic compounds, especially flavonoids.

BANKING MEMBERS:
Externa ao Programa - 2865332 - DALINE FERNANDES DE SOUZA ARAUJO
Externa à Instituição - JOVELINA SAMARA FERREIRA ALVES - USP
Presidente - 1490222 - SILVANA MARIA ZUCOLOTTO LANGASSNER