Banca de DEFESA: PEDRO IVO PALACIO LEITE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : PEDRO IVO PALACIO LEITE
DATE: 21/07/2020
TIME: 08:30
LOCAL: Videoconferência
TITLE:

Extraction of bioactive compounds from buriti (Mauritia flexuosa L.) fruit by eco-friendly supramolecular solvent: chemical and functional characterization

KEY WORDS:

Mauritia flexuosa L. Supramolecular. Euthetic solvents. Antioxidant. Carotenoids. Ultrasound.

PAGES: 117
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Buriti (Mauritia flexuosa L.) is a fruit that provides large quantities of bioactive components such as carotenoids and vitamin A. Thus, this species represents a raw material of great potential, due to the presence of bioactive compounds. In this sense, the objective of the present study was to extract the bioactive compounds from the peel and pulp of the buriti using green solvents to evaluate the antioxidant and antibacterial activities. Initially, extraction of the buriti peel and pulp was performed using deep euthectic solvents (DES), ethanol and supramolecular solvent (SUPRAS). In addition, the yield of carotenoid extraction with ethanol was evaluated by the conventional and ultrasound-assisted technique. The concentration of total carotenoids in the extracts was evaluated by spectrophotometry, and the concentration of total phenolic compounds and beta-carotene was determined by high-efficiency liquid chromatography (HPLC). The performance of SUPRAS extraction was compared with the results of ethanol extraction using the peel and buriti pulp as matrices. Beta-carotene extraction with SUPRAS yielded 5.82 ± 0.05 mg/g for peel and 26.7 ± 0.02 mg/g for pulp. For total phenolic compounds, yields were 32.1 ± 1.2 μg GAE/g for peel and 24.53 ± 4.9 μg GAE/g for pulp. All extracts presented excellent antioxidant activity, with emphasis on the extracts obtained with SUPRAS, which presented EC50 (concentration required to obtain a 50% antioxidant effect) for the sequestration of the ABTS radical of 3.00 μg/mL for the peel and 0.84 μg/mL for the pulp. These extract inhibiting bacterial growth of Staphylococcus aureus (ATCC 29213) and Enterococcus faecalis (ATCC 4028). Moreover, when associated with norfloxacin and gentamicin antibiotics, the extracts showed synergistic action against multiresistant strains of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. Thus, the extraction of bioactive compounds from buriti using a safe, biocompatible, biodegradable and environmentally friendly solvent such as SUPRAS represents an enormous potential for the development of new pharmaceutical, cosmetic and functional food products.

BANKING MEMBERS:
Externa à Instituição - ANA LÚCIA FIGUEIREDO PORTO - UFRPE
Externo ao Programa - 1346198 - EVERALDO SILVINO DOS SANTOS
Presidente - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Interno - 1714294 - MARCIO FERRARI