Banca de QUALIFICAÇÃO: LANNYA CLARA SANTOS TAVARES PESSOA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : LANNYA CLARA SANTOS TAVARES PESSOA
DATE: 06/02/2020
TIME: 14:00
LOCAL: Sala 1 do PPgCF
TITLE:

Preparation, characterization and in vitro assay of papain loaded solid lipid 3 nanoparticles

KEY WORDS:

Papain; 1,3-distearyl-2-oleyl glycerol; Solid lipid nanoparticles; Modified biomolecule release.

PAGES: 59
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Papain is a proteolytic enzyme with important medicinal properties. It is used both in wound care and in other diseases due to its proven anti-inflammatory effect. However, it is a protein that has limitations, such as low stability and high sensitivity under stress conditions. The enzyme immobilization means appears as a powerful strategy for overcoming these limitations. Nanotechnology can be considered as an alternative due to its advantages, such as, pay loading and transport of drugs and biomolecules. Nanocarriers improve stability, ability to cross biological barriers, and targeting of these molecules. The aim of the present study is preparing and monitoring physicochemical properties of solid lipid nanoparticles (SLN), using the triglyceride 1,3-distearyl-2-oleyl glycerol (TG1) as solid lipid for the modified release of papain. The solid lipid nanoparticles were produced by using solvent evaporation emulsification method. The papain was adsorbed in SLN. The chosen TG1 lipid matrix of plant origin has a healing pharmacological effect proven by the literature. Important physicochemical properties and parameters were assessed, such as the mean diameter, polydispersity index, zeta potential, atomic force microscopy (AFM), scanning electron microscopy (SEM), infrared absorption spectroscopy with fourier transform and reflectance attenuator accessory (FTIR-ATR), physical stability, and polyacrylamide gel electrophoresis. The in vitro studies with cell culture and antileishmanial activity were also performed. The protein-loaded NLS shown physical stability over 6 weeks with mean particle size less than 300 nm, and positive zeta potential. The FTIR-ATR spectroscopy showed band shifts of interaction between the compounds, which corroborates with gel electrophoresis that exhibited the presence of protein bands in the SLN. In addition, protein-loaded NLS did not affected the cell viability and improved the papain activity against the Leishimania genus. Therefore, SLN were successfully developed as an innovative strategy against the Leshimania ssp.

BANKING MEMBERS:
Interno - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Presidente - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA
Externa ao Programa - 2275877 - THAIS SOUZA PASSOS