Banca de QUALIFICAÇÃO: FRANCISCO LEANDRO MEDEIROS DE LUCENA JALES

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : FRANCISCO LEANDRO MEDEIROS DE LUCENA JALES
DATA : 28/10/2019
HORA: 08:30
LOCAL: SALA 2 DO PPGCF
TÍTULO:

ANTIVIRAL POTENTIAL OF Scoparia dulcis L. LEAF AGAINST HERPES SIMPLEX VIRUS TYPE 1 REPLICATION USING IN VITRO AND IN SILICO APPROACHES

PALAVRAS-CHAVES:

Scoparia dulcis, medicinal plants, HSV-1.

PÁGINAS: 102
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

The species Scoparia dulcis L. (Plantaginaceae), commonly called broom, is used in
folk medicine as healing, anti-inflammatory and gastrointestinal diseases. The main
components already described in the literature for the species are the titerpenoids. Thus,
the present study aimed to evaluate the phytochemical profile and biological potential as
antiviral of Scoparia leaf extract. dulcis L and toxicity. Hydroethanolic extracts were
prepared in the following ethanol proportions: 35% maceration (MAC 35), 70%
maceration (MAC 70), 35% turbolysis (TUR 35) and 70% turbolysis (TUR 70), which
were analyzed by Thin Layer Chromatography. and by liquid chromatography coupled
to mass spectrometer. In vitro cell viability test was performed on VERO cells by MTT
test exposed to different extracts. Subsequently, antiviral activity was evaluated and a
post-infection antiviral assay with HSV-1 virus (KOS strain) and an in silico study were
performed to evaluate possible targets of scopadulcic acid action. Acute toxicity was
assessed in an in vivo model by oral administration. Chromatographic analysis has so
far identified the flavonoids vicenin-2, vitexin, isovitexine and luteolin in S. dulcis
extracts. The triterpenes of the extract have not been characterized yet. In the MTT cell
viability assay, all extracts showed cell viability above 80% at concentrations below 125
ìg / mL. At a concentration of 250 ìg / mL TUR 35 extract showed significant
cytotoxicity with cell viability of only 48%. The other extracts presented viability above
80%. At concentrations of 500 and 1000 ìg / mL all extracts had viability below 40%.
In the antiviral models analyzed, the TUR 70 extract was the most efficient and is
considered promising for future evaluations. Finally, the in silico study showed a
possible interaction of scopadulcic acid with sirtuin 2, which may partly explain the
antiviral effect of the extracts.

MEMBROS DA BANCA:
Externo ao Programa - 2276354 - LEANDRO DE SANTIS FERREIRA
Presidente - 2275890 - MARCELO DE SOUSA DA SILVA
Externa ao Programa - 1801992 - PAULA RENATA LIMA MACHADO