Banca de QUALIFICAÇÃO: VALÉRIA COSTA DA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : VALÉRIA COSTA DA SILVA
DATA : 24/09/2019
HORA: 09:00
LOCAL: SALA 2 DO PPGCF
TÍTULO:

ATIVIDADE ANTI-INFLAMATÓRIA INTESTINAL DO EXTRATO AQUOSO DE Ipomoea asarifolia E AVALIAÇÃO DE TOXICIDADE IN VIVO

PALAVRAS-CHAVES:

intestinal inflammation; colitis; DNBS; rats; Ipomoea asarifolia; Convolvulaceae; toxicity; open field; rotarod.

PÁGINAS: 111
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Inflammatory bowel disease is triggered by an uncontrolled immune response associated with genetic, environmental, and intestinal microbiota imbalance. Its pharmacotherapy is based on drugs derived from aminosalicylates, corticosteroids, immunomodulators and biological agents, however, have serious adverse effects. Thus, the use of herbal medicines can be used as an adjunctive therapy in the treatment of IBD. For this, besides the use of assays that validate their pharmacological activities, there is a need for the use of toxicological assays, such as acute and subchronic toxicity assays safe use of these compounds. Ipomoea asarifolia, popularly known as “salsa” or “brave salsa”, belongs to the Convolvulaceae family, which is rich in bioactive compounds such as caffeic acid, chlorogenic acid and rutin. The aim of this approach was to study the preventive effect of aqueous extract of I. asarifolia (IA) in 2,4-dinitrobenzene sulfonic acid (DNBS)-induced colitis in rats and security validation. In acute and subchronic toxicity assays, single dose (2000 mg /kg) and serial doses were administered for 28 days (50, 100 and 200 mg /kg) of IA, respectively. Rats pretreated with IA extract (25,50 and 100 mg/kg) or sulfasalazine 250 mg/kg (SSZ) received intracolonic instillation of DNBS in 50% ethanol (v/v). No toxic effects were observed in vivo by hematological, hepatic, renal and motor changes. IA extract presented a protective effect against intestinal inflammation, with improvement in the disease activity index and macroscopic damage. IA or SSZ significantly reduced myeloperoxidase activity, and also down-regulation of the gene expression of JNK1, NF-êâ-p65, STAT3, and decreased levels of TNFá, IL-1â, and increased IL-10, associated with a significant improvement of oxidative stress, in addition to a reduction in MDA and an increase of glutathione in colonic tissue. The protective effect of the extract was also confirmed in histological evaluation, showing preservation of the colonic cytoarchitecture. Immunohistochemical analysis revealed down-regulation of NF-êâ-p65, iNOS, IL-17, and up-regulation of SOCs-1 and MUC-2. Given the results obtained, IA extract has intestinal antioxidant and anti-inflammatory properties and has been shown to have potential for possible application in the control of human IBD.

MEMBROS DA BANCA:
Presidente - 1789788 - ADLEY ANTONINI NEVES DE LIMA
Externo ao Programa - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externa ao Programa - 1720860 - VANESSA DE PAULA SOARES RACHETTI