Banca de QUALIFICAÇÃO: WENDELL MEDEIROS DE AZEVEDO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : WENDELL MEDEIROS DE AZEVEDO
DATA : 29/08/2019
HORA: 14:00
LOCAL: SALA 2 DO PPGCF
TÍTULO:

Lipase production by Aspergillus terreus NRRL-255: Evaluation of the inducing effect of cacay (Caryodendron orinocense), optimization and biochemical characterization

PALAVRAS-CHAVES:

Antioxidant capacity; antibacterial activity; Aspergillus terreus; solid state fermentation (SSF).

PÁGINAS: 112
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

The cacay (Caryodendron orinocense Karst.) is a typical Amazonian tree that grows along the Andes. Its seeds have a rich nutritional composition and high potential for industrial exploitation. The present work aims to evaluate the chemical, physicochemical, and bioactive properties of cacay oil and butter and the potential inducer to lipase production from Aspergillus terreus NRRL - 255 by solid state fermentation (SSF). Initially, the chemical and physicochemical characterization were performed, as well as the determination of antioxidant capacity and antibacterial activity. In addition, agro-industrial wastes were characterized for use as non-inert solid supports by SSF. The potential of the lipid inducer for fungal lipase production was also evaluated, and the lipase production process was optimized by experimental design. Finally, the lipases produced were characterized by the influence of pH, temperature, metal ions, chelating agent, and surfactants. Regarding the chemical and physicochemical characteristics, it is suggested that the cacay oil presents a high number of unsaturations due to its high iodine value (116.35 cg I2.g-1). In contrast, the butter presented greater stability against lipid peroxidation (7.77 meq.kg-1) and high saturated fatty acids content (76.51%). The hydrophilic fraction of cacay oil was highlighted in the quantification of total phenolic compounds (326.27 mg EAG.kg-1) and antioxidant capacity in vitro (156.57 μM TE.g-1), by DPPH radical scavenging assay. The cacay oil showed antibacterial activity against the bacterial strains of Klebsiella pneumoniae (66.20%) and Bacillus cereus (44.99%). The present study found favorable results for wheat bran as a non-inert solid substrate to lipase production, such as the high water absorption index (3.65 g H2O.g-1). The cacay butter presented the highest lipolytic activity (308.14 U.g-1) in screening of lipid inducer. After optimization of lipase production by experimental design, temperature was a significant independent variable with a critical value of 27.36 °C and maximum enzimatic activity of 2,867.18 U.g-1. Regarding the lipase characterization, a maximum relative activity was found at pH 7.0 and temperature of 35 ºC. An inhibitory effect was observed for the Ca2+, Mn2+, Zn2+, Fe2+, and Cu2+ ions. The lipase activity was not significantly affected in the presence of EDTA. On the other hand, increase of lipase activity was found with the reduction of SDS concentration and increase of Triton X-100. Finally, cacay oil presented bioactive properties which might justify its use as a bioactive in cosmetic formulations. On the other hand, the use of cacay butter as a lipid inducer showed a high lipolytic activity, indicating a potential biotechnology to produce industrial lipases.

MEMBROS DA BANCA:
Externo ao Programa - 1346198 - EVERALDO SILVINO DOS SANTOS
Externa ao Programa - 3211846 - KARLA SUZANNE FLORENTINO DA SILVA CHAVES DAMASCENO
Presidente - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA