Banca de DEFESA: JULIA MORAIS FERNANDES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : JULIA MORAIS FERNANDES
DATA : 26/07/2019
HORA: 08:30
LOCAL: SALA DE VIDEOCONFERÊNCIA POP-RN (Campus Central/UFRN)
TÍTULO:

Kalanchoe laciniata and Bryophyllum pinnatum: an updated reviewabout ethnopharmacology, phytochemistry, pharmacology andtoxicologyJúlia

PALAVRAS-CHAVES:

Crassulaceae; metabolomic; chemical markers; herbal medicine.

PÁGINAS: 306
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Kalanchoe laciniata (L.) DC. and Bryophillum pinnatum (Lam) Pers. (Crassulaceae)
known populary as saião or coirama are used in Brazil, mainly in the Northeast region, and
their use remarks in the anti-inflammatory problems treatment. Regarding chemical
composition, patuletin, kaempferol and quercetin glycosylates derivates are the main
compounds decribed, however the active compounds are not known. Besides, there are not
enough control quality studies that can be contribute to authentication and differentiation
between K. laciniata and B. pinnatum, especially by High or Ultra Performance Liquid
Chromatography (HPLC or UPLC) coupled Mass Spectrometer (MS) or Nuclear Magnetic
Resonance (NMR). Once B. pinnatum is in Renisus (Relação Nacional de Plantas Medicinais
de Interesse do SUS), it is necessary to increase studies with the both species to ensure safety,
efficacy and quality of the possible herbal drug can be developed and distributed by SUS. In
this context, the proposal is performed a metabolomic and pharmacologic approach with K.
laciniata and B. pinnatum species. To achieve this goal, the methodology was divided: i:
isolation and characterization of main compounds of leaves extracts of both species; ii:
quantification of chemical markers; iii: metabolomic approach of the extracts in response to
stress induced by drought and salt stress; iv: in vitro assays to evaluate the inhibitory effects of
the extracts against the enzymes that trigger the B. erythromelas envenomation. The most
significant results were i. by the centrifugal partition chromatography (CPC) technique
application, it has enabled the isolation of substance not described for the genus, named
sarmentosin; ii. a) to quantify three flavonoids in the extract of B. pinnatum by HPLC-DAD
and to suggest the flavonoid quercetin 3-O-α-L-arabinopyranosyl-(1→2)-O-α-Lrhamnopyranoside
as a specific marker it, and b) to develop and validate an NMR methodology
to quantify sarmentosin in the extract of B. pinnatum; iii. metabolomic approach obtained by
HPLC-MS/MS data showed that drought and salinity conditions can influence the expression
of different metabolites, indicating that both species are resistant to watering suspension up to
the 20th day, but that the lower amount of salinity in the irrigation water already induces
differences in metabolic expression; iv. in vitro activities showed the potential of the species in
the face of poisoning and showed that saline and water stresses conditions directly influence
PLA2 activity. In this context, this work represented a chemical and pharmacological advance
in the study of these species, regarding the development of an efficient methodology for the
isolation of main compunds in large quantity, besides suggesting certain culture conditions that
may influence the expression of metabolites that may be linked to the biological activities of
the extracts.

MEMBROS DA BANCA:
Interno - 1492900 - CICERO FLAVIO SOARES ARAGAO
Externo à Instituição - FERNANDO BATISTA COSTA - USP
Externa à Instituição - MAGDA RHAYANNY ASSUNCAO FERREIRA - UFPE
Interna - 1871916 - RAQUEL BRANDT GIORDANI
Presidente - 1490222 - SILVANA MARIA ZUCOLOTTO LANGASSNER