Banca de DEFESA: JÚLIA GABRIELA RAMOS PASSOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : JÚLIA GABRIELA RAMOS PASSOS
DATA : 29/03/2019
HORA: 08:00
LOCAL: SALA 7B2 DO DFAR
TÍTULO:

EVALUATION OF THE ANTI-INFLAMMATORY AND ANTIOPHIDIC ACTIVITY OF A GEL CONTAINING THE HYDROETHANOL EXTRACT OF Jatropha mollissima (Pohl) Baill.

PALAVRAS-CHAVES:

Jatropha. Jatropha mollissima. Bothrops jararaca. Snakebites. Serpents. Anti-inflammatory. Healing.

PÁGINAS: 156
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Accidents caused by snakes are considered a major public health problem in several regions of the world. Bothrops are responsible for the vast majority of ophidic accidents in Brazil. It is known that, currently, the only specific treatment available for the bite of these snakes is the bothropic antivenom, but it has some limitations, such as not combat local effects effectively, difficult access in some regions, and high cost. Plants of the genus Jatropha (Euphorbiaceae) are often indicated by traditional medicine, in some regions of Brazil, to reverse the damage caused by snake bites and also with anti-inflammatory function. In this view, the objective of this work was to evaluate the anti-inflammatory, healing and antiophidic potential against the venom of the snake Bothrops jararaca of the hydroethanolic extract of the leaves of Jatropha mollissima (Pohl) Baill. incorporated into a formulation developed for topical application. Tests were performed to evaluate the topical anti-inflammatory activity of this species in carrageenan-induced paw edema and ear edema induced by single application (acute inflammation) or multiple applications (chronic inflammation) of chroton oil. Tests were also performed to evaluate the healing activity of the formulation, while the skin irritation test was conducted to analyze safety during topical use of the formulations developed. It was possible to observe that the gel had anti-inflammatory action in acute inflammation models induced by carrageenan, and in acute and chronic inflammation models by chroton oil. chroton oil. It was also observed that the gel has cicatrizant activity, since it significantly decreased the size of the wound in relation to the placebo group. The gel did not cause skin irritation in mice, proving to be safe for topical use. The efficacy of the gel containing the hydroethanolic extract of the leaves of J. mollissima alone and in association with the commercial polyvalent antibothropic-crotalic commercial antivenom in relation to its antiophidic potential against the local effects of B. jararaca envenoming were evaluated in the paw edema and muscle hemorrhagic models and it was seen that the venom-induced edematogenic and hemorrhagic activities were inhibited, both by the gel alone and by the association with the antiophidic antivenom, since these treatments reduced edema (the gel treatment containing the extract in association with the antiophidic antivenom had an inhibition of approximately 90% after four hours of the envenoming), in addition to significantly reducing the concentration of hemoglobin in the evaluated tissues, consequently having a significant anti-hemorrhagic potential. In conclusion, the results show that the gel containing the hydroethanolic extract of the leaves of J. mollissima shows potential anti-inflammatory, healing and antiophidic activity, acting on the local effects of the bite, suggesting that this formulation of topical use has an important antiophidic potential as a complementary alternative to the treatment of local effects caused by bothropic envenoming, besides acting as healing potential and topical anti-inflammatory for the treatment of various skin diseases.

MEMBROS DA BANCA:
Interna - 2330188 - GERLANE COELHO BERNARDO GUERRA
Externa à Instituição - JULIANA FÉLIX DA SILVA - UNIFACEX
Externa à Instituição - KARLA PATRÍCIA DE OLIVEIRA LUNA - UEPB
Presidente - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA