Banca de DEFESA: FRANCISCO DE ASSIS BEZERRA NETO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : FRANCISCO DE ASSIS BEZERRA NETO
DATA : 26/03/2019
HORA: 14:00
LOCAL: Sala de videoconferência do Centro de estudos da MEJC
TÍTULO:

Candida spp. adherence to HBEC in the presence of the saponin-enriched fraction of Ziziphus joazeiro

PALAVRAS-CHAVES:

Candida spp., virulence factors, candidemia, sinthethic antifungals, Ziziphus joazeiro, saponins.

PÁGINAS: 191
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Bloodstream infections represent an important public health problem, with attributed mortality of 40-60%. In addition to their known in vitro virulence potential, Candida spp. have been drawing attention to the growing number of reports of resistance to antifungal drugs, representing a serious problem for the treatment of infections caused by these microorganisms. This study aimed to evaluate the antifungal susceptibility of Candida spp. from patients with Candida bloodstream infection against synthetic antifungal compounds, as well as an enriched fraction obtained from the extract of Ziziphus joazeiro. We also investigated the interference of this natural product on the expression of Candida spp. virulence factors. Material and methods: Seventy Candida spp. isolates from blood cultures were analyzed. The microdilution broth technique was performed to investigate the susceptibility of the isolates against synthetic antifungals. Subsequently, 50 isolates were selected and the evaluation of the antifungal activity of the fraction was performed. A total of 27 isolates were selected for virulence factor expression assays in the absence and presence of the fraction. None of the isolates presented resistance to amphotericin B and caspofungin. Four isolates of C. albicans and 1 isolate of C. glabrata were resistant to fluconazole, whereas 3 isolates of C. albicans, 1 of C. tropicalis and 2 of C. parapsilosis complex showed dose-dependent susceptibility to this drug. Four isolates of C. albicans and 1 of C. tropicalis showed a dose-dependent susceptibility to itraconazole. The isolates identified as C. albicans and C. glabrata were susceptible to the fraction with MFCs ranging from 5000-10000 μg/mL. The strains treated with the fraction showed a reduction of about 26% in the capacity of adhesion buccal epithelial cells, a lower production of phospholipase, haemolisyns and a decrease in filamentation capacity. The enriched fraction of Z. joazeiro presents significant antifungal activity, being able to interfere with the expression of virulence factors in vitro of this group of yeasts. Further studies should be performed in order to evaluate the mechanisms of action involved.

MEMBROS DA BANCA:
Externa ao Programa - 1149398 - EVELINE PIPOLO MILAN
Presidente - 1715308 - GUILHERME MARANHAO CHAVES
Externo à Instituição - REGINALDO GONÇALVES DE LIMA NETO - UFPE