Banca de DEFESA: FIAMMA GLÁUCIA DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : FIAMMA GLÁUCIA DA SILVA
DATA : 14/12/2018
HORA: 08:30
LOCAL: SALA 2 DO PPGCF
TÍTULO:

A BIOTECHNOLOGICAL APPROACH TO IMMUNOTHERAPY: ANTIVENOM AGAINST Crotalus durissus cascavella SNAKE VENOM PRODUCED FROM BIODEGRADABLE NANOPARTICLES

PALAVRAS-CHAVES:

Antivenom. Crotalus durissus cascavella. Chitosan Nanoparticles.

PÁGINAS: 72
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Snakebite envenoming is a tropical disease neglected worldwide. In Brazil, the Crotalus durissus cascavella (CDC) snake belongs to a genus with venom of highest lethality. A search for new immunoadjuvants aimed to expand the therapeutic alternatives to improve vaccines and antivenom. This approach proposed to produce small and narrow-sized cationic CDC venom-loaded chitosan nanoparticles (CHNP), by the adsorption and incorporation method, able to induce antibody response against the CDC venom. The ionic gelation method induced the formation of stable and slightly smooth spherical nanoparticles (< 160 nm) with protein loading efficiency superior to 90%. The interactions between venom proteins and CHNP assessed using Fourier transform infrared spectroscopy corroborated with the in vitro release behavior of proteins from nanoparticles. Finally, the immunization animal model using BALB/c mice demonstrated the higher effectiveness of CDC venom-loaded CHNP, mainly by the adsorption method, compared to aluminum hydroxide, a conventional immunoadjuvant. Thus, CHNPs loaded with CDC venom exhibited a promising biotechnological approach to immunotherapy.

MEMBROS DA BANCA:
Interno - 1639820 - ARNOBIO ANTONIO DA SILVA JUNIOR
Presidente - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA
Externo ao Programa - 1801992 - PAULA RENATA LIMA MACHADO
Externo à Instituição - ROBERTO NICOLETE