Banca de QUALIFICAÇÃO: RAQUEL RIBEIRO BARBOSA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : RAQUEL RIBEIRO BARBOSA
DATA : 27/09/2018
HORA: 09:00
LOCAL: Sala 1 do PPgCF
TÍTULO:

Anthelmintic effect of hidroetanolic extracts from leaves Calotropis procera against sheep gastrointestinal nematodes

PALAVRAS-CHAVES:

Apocynacea, “silk flower”, phytotherapy, nematodes, veterinary

PÁGINAS: 109
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

One of the major obstacles of ovinocaprinocultura in Northeast Brazil is parasitism by gastrointestinal nematodes. The occurrence of anthelmintic resistance in herds of this region has favored investigations on the use of alternative methods, such as the use of medicinal plants. Reports of popular use have motivated research to scientifically prove the anthelmintic potential of Calotropis procera through in vitro and in vivo studies. The objective of this study was to evaluate the anthelmintic potential of C. procera (ECp) leaves by in vitro and in vivo assays on gastrointestinal nematodes of naturally infected sheep. The experiment was divided into stages: I. Plant phytochemical screening; II. Evaluation of anthelmintic activity in vitro: egg hatching inibition test-EHI and larval development test -LDT; III. Toxicity assessment: MTT assay and acute toxicity test in rats; IV. Clinical trial: reduction test of egg count in faeces in sheep. As results, by means of the phytochemical screening it was possible to detect the presence of some groups of secondary metabolites: flavonoids, saponins, alkaloids and phenols. Thin layer chromatography may indicate the presence of rutin, camferol, quercetin and luteolin. In the evaluation of in vitro efficacy, ECp showed efficacy greater than 90% in concentrations of 24 to 72 mg/mL (100%) after 48 h of incubation in EHI. All the concentrations evaluated differed statistically from the negative control (distilled water) p <0.0001. In LDT, a significant difference was observed between the concentrations evaluated and the negative control at doses above 6 mg/mL with values of p = 0.000. The comparison between the concentrations of the extract and the positive control (ivermectin) showed a significant difference for the doses of 1, 12, 24 and 36 mg/mL with values of p = 0.000. The MTT assay showed that extracts at concentrations of 500 and 1.000 μg/mL differed significantly from the 0% DMSO negative control, as well as from the 2% DMSO control in the Tukey test (p <0.05). No significant difference (p> 0.05) was observed between 1% DMSO and the sample at 500 μg / mL, nor between DMSO 2% and the sample at 1000 μg/mL by the t test, which suggests that the toxicity presented associated with the presence of DMSO in the sample. Concentrations of 1 to 250 μg/mL did not present cytotoxicity when compared to the negative control. In the acute toxicity test in rats, no mortality was observed at doses of 1.000 and 2.000 mg/Kg orally. No symptoms of intoxication were observed in the first 24 hours posttreatment, nor significant changes in the blood count after 14 days of observation. No significant difference was observed in the dosages of AST, ALT, urea, creatinine and alkaline phosphatase of the treatments when compared to the control. A significant difference (p <0.05) was observed between the treatments (1.000 and 2.000 mg / kg) when compared to the control with respect to the quantification of total proteins. The group receiving the dose of 2.000 mg/Kg differed statistically from the control group regarding albumin dosage (p <0.05). The preliminary clinical trial was carried out at the Ipanguaçu-RN campus of the Federal Institute of Education Science and Technology of Rio Grande do Norte (IFRN), with a total of 15 sheep divided into three groups (n = 5), the results demonstrated that the extract of C. procera when administered at a dose of 300 mg/Kg orally reduced the number of epg in the animals on 25 days and 26.52% on day 14 and day 28 after treatment, respectively.

MEMBROS DA BANCA:
Interno - 2087759 - ANDRE DUCATI LUCHESSI
Externo ao Programa - 1714262 - LILIAN GIOTTO ZAROS DE MEDEIROS
Presidente - 2275890 - MARCELO DE SOUSA DA SILVA