Banca de QUALIFICAÇÃO: ALAINE MARIA DOS SANTOS SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : ALAINE MARIA DOS SANTOS SILVA
DATA : 07/08/2018
HORA: 09:00
LOCAL: SALA 1 DO PPGCF
TÍTULO:

Fluorescent labeled benznidazole-loading biodegradable nanoparticles
to improve drug targeting in infected cells with parasites

PALAVRAS-CHAVES:

Benznidazole. Modified release. Polymeric nanoparticles. Fluorescent probe. Chaga’s disease.

PÁGINAS: 135
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Benznidazole (BNZ) is the drug of choice for the treatment of patients with Chaga’s disease. Despite its wide use, it presents problems of efficacy due to the high toxicity, difficulty of crossing the biological barriers, besides the low solubility in aqueous medium. Nanoparticles have a proven ability to cross biological barriers and intracellular drug targeting, especially when surface binders are inserted to increase drug targeting for infected cells. The aim of the present work is to develop functionalized and fluorescent nanoparticles of poly (lactide-co-glycolide) (PLGA) for modified release of benznidazole. The particles were produced by the emulsification-solvent evaporation method. The standardization of the formulation and the parameters of the preparation were monitored by measurements of average particle size, polydispersity index, zeta potential, atomic force microscopy (AFM), scanning electron microscopy (SEM), confocal microscopy (CM), attenuated total reflectance fourier transforms infrared spectroscopy (ATR–FTIR), thermogravimetric analysis (TGA), encapsulation efficiency and in vitro studies. Stable and spherical polymeric nanoparticles below 300 nm were optimized, with encapsulation efficiency greater than 95%. In vitro release kinetics of the drug demonstrated a slow release adjusted by mathematical models. Cardiac myoblastic cells (H9c2) demonstrated the biocompatibility of nanoparticles and the improvement of the biological activity of BNZ nanoparticles. Assays made with normal cells (Hek 293), tumor cells (Hep G2 and HT-29), epimastigotes (Y, CL-Brenner and Dm28c strains) and amastigotes (H9c2 and Dm28c strain) show an increase in the potency of the nanoparticles over free BNZ. Confocal microscopy analysis, in which a fluorescent probe was incorporated to verify at different times the targeting, show that the nanoparticles enter the parasite with high efficiency. Thus, the present work systematically addresses the development of a novel nanotechnological system with innovative potential for increasing the efficacy of benznidazole in infected cells.

MEMBROS DA BANCA:
Interno - 1218940 - ANTONIA CLAUDIA JACOME DA CAMARA
Externo ao Programa - 1639676 - EDSON NORIYUKI ITO
Externo à Instituição - HENRIQUE RODRIGUES MARCELINO - UFBA
Presidente - 2275890 - MARCELO DE SOUSA DA SILVA